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Attention Score in Context
| Title |
The "one-step" Bean pod mottle virus (BPMV)-derived vector is a functional genomics tool for efficient overexpression of heterologous protein, virus-induced gene silencing and genetic mapping of BPMV R-gene in common bean (Phaseolus vulgaris L.)
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|---|---|
| Published in |
BMC Plant Biology, August 2014
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| DOI | 10.1186/s12870-014-0232-4 |
| Pubmed ID | |
| Authors |
Stéphanie Pflieger, Sophie Blanchet, Chouaib Meziadi, Manon MS Richard, Vincent Thareau, Fanny Mary, Céline Mazoyer, Valérie Geffroy |
| Abstract |
BackgroundOver the last two years, considerable advances have been made in common bean (Phaseolus vulgaris L.) genomics, especially with the completion of the genome sequence and the availability of RNAseq data. However, as common bean is recalcitrant to stable genetic transformation, much work remains to be done for the development of functional genomics tools adapted to large-scale studies.ResultsHere we report the successful implementation of an efficient viral vector system for foreign gene expression, virus-induced gene silencing (VIGS) and genetic mapping of a BPMV resistance gene in common bean, using a ¿one-step¿ BPMV vector originally developed in soybean. With the goal of developing this vector for high-throughput VIGS studies in common bean, we optimized the conditions for rub-inoculation of infectious BPMV-derived plasmids in common bean cv. Black Valentine. We then tested the susceptibility to BPMV of six cultivars, and found that only Black Valentine and JaloEEP558 were susceptible to BPMV. We used a BPMV-GFP construct to detect the spatial and temporal infection patterns of BPMV in vegetative and reproductive tissues. VIGS of the PHYTOENE DESATURASE (PvPDS) marker gene was successfully achieved with recombinant BPMV vectors carrying fragments ranging from 132 to 391 bp. Finally, we mapped a gene for resistance to BPMV (R-BPMV) at one end of linkage group 2, in the vicinity of a locus (I locus) previously shown to be involved in virus resistance.ConclusionsThe ¿one-step¿ BPMV vector system therefore enables rapid and simple functional studies in common bean, and could be suitable for large-scale analyses. In the post-genomic era, these advances are timely for the common bean research community. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 2% |
| France | 1 | 2% |
| Unknown | 50 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 19% |
| Researcher | 10 | 19% |
| Student > Bachelor | 5 | 10% |
| Professor > Associate Professor | 4 | 8% |
| Student > Master | 4 | 8% |
| Other | 11 | 21% |
| Unknown | 8 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 37 | 71% |
| Biochemistry, Genetics and Molecular Biology | 6 | 12% |
| Unspecified | 1 | 2% |
| Energy | 1 | 2% |
| Unknown | 7 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 May 2015.
All research outputs
#18,376,927
of 22,761,738 outputs
Outputs from BMC Plant Biology
#2,083
of 3,236 outputs
Outputs of similar age
#168,260
of 236,210 outputs
Outputs of similar age from BMC Plant Biology
#31
of 61 outputs
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So far Altmetric has tracked 3,236 research outputs from this source. They receive a mean Attention Score of 3.0. This one is in the 22nd percentile – i.e., 22% of its peers scored the same or lower than it.
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