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Mendeley readers
Attention Score in Context
| Title |
Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks
|
|---|---|
| Published in |
Microbial Cell Factories, September 2014
|
| DOI | 10.1186/s12934-014-0127-y |
| Pubmed ID | |
| Authors |
Zharain Bawa, Sarah J Routledge, Mohammed Jamshad, Michelle Clare, Debasmita Sarkar, Ian Dickerson, Markus Ganzlin, David R Poyner, Roslyn M Bill |
| Abstract |
Pichia pastoris is a widely-used host for recombinant protein production; expression is typically driven by methanol-inducible alcohol oxidase (AOX) promoters. Recently this system has become an important source of recombinant G protein-coupled receptors (GPCRs) for structural biology and drug discovery. The influence of diverse culture parameters (such as pH, dissolved oxygen concentration, medium composition, antifoam concentration and culture temperature) on productivity has been investigated for a wide range of recombinant proteins in P. pastoris. In contrast, the impact of the pre-induction phases on yield has not been as closely studied. In this study, we examined the pre-induction phases of P. pastoris bioreactor cultivations producing three different recombinant proteins: the GPCR, human A(2a) adenosine receptor (hA(2a)R), green fluorescent protein (GFP) and human calcitonin gene-related peptide receptor component protein (as a GFP fusion protein; hCGRP-RCP-GFP). |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 50% |
| United Kingdom | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 83 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 1% |
| Mexico | 1 | 1% |
| India | 1 | 1% |
| Unknown | 80 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 17 | 20% |
| Student > Ph. D. Student | 17 | 20% |
| Researcher | 15 | 18% |
| Student > Bachelor | 12 | 14% |
| Student > Doctoral Student | 3 | 4% |
| Other | 10 | 12% |
| Unknown | 9 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 34 | 41% |
| Agricultural and Biological Sciences | 18 | 22% |
| Engineering | 6 | 7% |
| Chemistry | 3 | 4% |
| Chemical Engineering | 3 | 4% |
| Other | 9 | 11% |
| Unknown | 10 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 July 2021.
All research outputs
#6,273,105
of 22,763,032 outputs
Outputs from Microbial Cell Factories
#416
of 1,595 outputs
Outputs of similar age
#61,911
of 237,916 outputs
Outputs of similar age from Microbial Cell Factories
#10
of 28 outputs
Altmetric has tracked 22,763,032 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 1,595 research outputs from this source. They receive a mean Attention Score of 4.4. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 237,916 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 28 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.