Title |
Capturing needles in haystacks: a comparison of B-cell receptor sequencing methods
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Published in |
BMC Immunology, August 2014
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DOI | 10.1186/s12865-014-0029-0 |
Pubmed ID | |
Authors |
Rachael JM Bashford-Rogers, Anne L Palser, Saad F Idris, Lisa Carter, Michael Epstein, Robin E Callard, Daniel C Douek, George S Vassiliou, George A Follows, Mike Hubank, Paul Kellam |
Abstract |
Deep-sequencing methods are rapidly developing in the field of B-cell receptor (BCR) and T-cell receptor (TCR) diversity. These promise to revolutionise our understanding of adaptive immune dynamics, identify novel antibodies, and allow monitoring of minimal residual disease. However, different methods for BCR and TCR enrichment and amplification have been proposed. Here we perform the first systematic comparison between different methods of enrichment, amplification and sequencing for generating BCR and TCR repertoires using large sample numbers. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 25% |
Unknown | 3 | 75% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 75% |
Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 5 | 3% |
United Kingdom | 3 | 2% |
Russia | 3 | 2% |
Germany | 1 | <1% |
Unknown | 146 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 51 | 32% |
Student > Ph. D. Student | 35 | 22% |
Student > Bachelor | 10 | 6% |
Student > Master | 10 | 6% |
Other | 7 | 4% |
Other | 23 | 15% |
Unknown | 22 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 45 | 28% |
Immunology and Microbiology | 29 | 18% |
Biochemistry, Genetics and Molecular Biology | 28 | 18% |
Medicine and Dentistry | 15 | 9% |
Engineering | 5 | 3% |
Other | 9 | 6% |
Unknown | 27 | 17% |