Title |
Genome wide screening of RNAi factors of Sf21 cells reveal several novel pathway associated proteins
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Published in |
BMC Genomics, September 2014
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DOI | 10.1186/1471-2164-15-775 |
Pubmed ID | |
Authors |
Subhanita Ghosh, Pavan Kumar Kakumani, Ajit Kumar, Pawan Malhotra, Sunil K Mukherjee, Raj K Bhatnagar |
Abstract |
RNA interference (RNAi) leads to sequence specific knock-down of gene expression and has emerged as an important tool to analyse gene functions, pathway analysis and gene therapy. Although RNAi is a conserved cellular process involving common elements and factors, species-specific differences have been observed among different eukaryotes. Identification of components for RNAi pathway is pursued intensively and successful genome-wide screens have been performed for components of RNAi pathways in various organisms. Functional comparative genomics analysis offers evolutionary insight that forms basis of discoveries of novel RNAi-factors within related organisms. Keeping in view the academic and commercial utility of insect derived cell-line from Spodoptera frugiperda, we pursued the identification and functional analysis of components of RNAi-machinery of Sf21 cell-line using genome-wide application. |
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Country | Count | As % |
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India | 1 | 14% |
France | 1 | 14% |
Unknown | 5 | 71% |
Demographic breakdown
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Members of the public | 4 | 57% |
Scientists | 3 | 43% |
Mendeley readers
Geographical breakdown
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France | 1 | 2% |
Unknown | 49 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 10 | 20% |
Student > Master | 10 | 20% |
Researcher | 6 | 12% |
Student > Doctoral Student | 5 | 10% |
Student > Bachelor | 3 | 6% |
Other | 9 | 18% |
Unknown | 7 | 14% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 26 | 52% |
Biochemistry, Genetics and Molecular Biology | 12 | 24% |
Medicine and Dentistry | 2 | 4% |
Unspecified | 1 | 2% |
Unknown | 9 | 18% |