A round robin approach to the analysis of bisphenol a (BPA) in human blood samples

Laura N Vandenberg, Roy R Gerona, Kurunthachalam Kannan, Julia A Taylor, Richard B van Breemen, Carrie A Dickenson, Chunyang Liao, Yang Yuan, Retha R Newbold, Vasantha Padmanabhan, Frederick S vom Saal, Tracey J Woodruff

Human exposure to bisphenol A (BPA) is ubiquitous, yet there are concerns about whether BPA can be measured in human blood. This Round Robin was designed to address this concern through three goals: 1) to identify collection materials, reagents and detection apparatuses that do not contribute BPA to serum; 2) to identify sensitive and precise methods to accurately measure unconjugated BPA (uBPA) and BPA-glucuronide (BPA-G), a metabolite, in serum; and 3) to evaluate whether inadvertent hydrolysis of BPA-G occurs during sample handling and processing.