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Two dimensional gel phosphoproteome of peripheral blood mononuclear cells: comparison between two enrichment methods

Overview of attention for article published in Proteome Science, September 2014
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Title
Two dimensional gel phosphoproteome of peripheral blood mononuclear cells: comparison between two enrichment methods
Published in
Proteome Science, September 2014
DOI 10.1186/s12953-014-0046-1
Pubmed ID
Authors

Maria Teresa Rocchetti, Michela Alfarano, Leonarda Varraso, Salvatore Di Paolo, Massimo Papale, Elena Ranieri, Giuseppe Grandaliano, Loreto Gesualdo

Abstract

Protein phosphorylation is considered a key event in signal transduction. Peripheral blood mononuclear cells (PBMCs) are a critical component of the immune system. The analysis of PBMCs phosphoproteome might help elucidate the signaling pathways essential to their biological role in health, immunological diseases and cancer. Enrichment of phosphoproteins becomes a prerequisite for phosphoproteome analysis and conventionally requires a multi-step procedure and sophisticated equipments. In this study, we standardized 2D-PAGE phosphoproteome analysis of PBMCs and compared two phosphoprotein enrichment methods, lanthanum chloride precipitation and affinity micro-column. Further, the different specificity for PBMCs phosphorylated proteins of each method was investigated.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 27%
Student > Ph. D. Student 3 14%
Student > Postgraduate 2 9%
Professor > Associate Professor 2 9%
Student > Bachelor 1 5%
Other 4 18%
Unknown 4 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 27%
Agricultural and Biological Sciences 4 18%
Medicine and Dentistry 4 18%
Immunology and Microbiology 3 14%
Unknown 5 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 September 2014.
All research outputs
#17,726,563
of 22,763,032 outputs
Outputs from Proteome Science
#124
of 190 outputs
Outputs of similar age
#160,643
of 238,628 outputs
Outputs of similar age from Proteome Science
#1
of 1 outputs
Altmetric has tracked 22,763,032 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 190 research outputs from this source. They receive a mean Attention Score of 2.7. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
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