Title |
Two dimensional gel phosphoproteome of peripheral blood mononuclear cells: comparison between two enrichment methods
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Published in |
Proteome Science, September 2014
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DOI | 10.1186/s12953-014-0046-1 |
Pubmed ID | |
Authors |
Maria Teresa Rocchetti, Michela Alfarano, Leonarda Varraso, Salvatore Di Paolo, Massimo Papale, Elena Ranieri, Giuseppe Grandaliano, Loreto Gesualdo |
Abstract |
Protein phosphorylation is considered a key event in signal transduction. Peripheral blood mononuclear cells (PBMCs) are a critical component of the immune system. The analysis of PBMCs phosphoproteome might help elucidate the signaling pathways essential to their biological role in health, immunological diseases and cancer. Enrichment of phosphoproteins becomes a prerequisite for phosphoproteome analysis and conventionally requires a multi-step procedure and sophisticated equipments. In this study, we standardized 2D-PAGE phosphoproteome analysis of PBMCs and compared two phosphoprotein enrichment methods, lanthanum chloride precipitation and affinity micro-column. Further, the different specificity for PBMCs phosphorylated proteins of each method was investigated. |
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Student > Ph. D. Student | 3 | 14% |
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Student > Bachelor | 1 | 5% |
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Unknown | 5 | 23% |