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Attention Score in Context
| Title |
GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots
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|---|---|
| Published in |
BMC Plant Biology, September 2014
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| DOI | 10.1186/s12870-014-0245-z |
| Pubmed ID | |
| Authors |
Li Chen, Bingjun Jiang, Cunxiang Wu, Shi Sun, Wensheng Hou, Tianfu Han |
| Abstract |
BackgroundPromoters play important roles in gene expression and function. There are three basic types of promoters: constitutive, specific, and inducible. Constitutive promoters are widely used in genetic engineering, but these promoters have limitations. Inducible promoters are activated by specific inducers. Tissue-specific promoters are a type of specific promoters that drive gene expression in specific tissues or organs. Here, we cloned and characterized the GmPRP2 promoter from soybean. The expression pattern indicated that this promoter is root-preferential in transgenic Arabidopsis and the hairy roots of soybean. It can be used to improve the root resistance or tolerance to pathogens, pests, malnutrition and other abiotic stresses which cause extensive annual losses in soybean production.ResultsThe GmPRP2 promoter (GmPRP2p-1062) was isolated from soybean cv. Williams 82. Sequence analysis revealed that this promoter contains many cis-acting elements, including root-specific motifs. The GmPRP2p-1062 and its 5¿-deletion fragments were fused with the GUS reporter gene and introduced into Arabidopsis and the hairy roots of soybean to further determine promoter activity. Histochemical analysis in transgenic Arabidopsis showed that GUS activity was mainly detected in roots and hypocotyls in all deletion fragments except GmPRP2p-471 (a 5¿-deletion fragment of GmPRP2p-1062 with 471 bp length). GUS activity was higher in transgenic Arabidopsis and hairy roots with GmPRP2p-1062 and GmPRP2p-852 (a 5¿-deletion fragment of GmPRP2p-1062 with 852 bp length) constructs than the other two constructs. GUS activity was enhanced by NaCl, PEG, IAA and JM treatments and decreased by SA, ABA and GA treatments in transgenic Arabidopsis.ConclusionsGmPRP2p-1062 is a root-preferential promoter, and its core fragment for root-preferential expression might lie between ¿369 and +1. GmPRP2p-852 may be useful in the genetic engineering of novel soybean cultivars in the future. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| South Africa | 1 | 1% |
| Unknown | 70 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 17% |
| Researcher | 11 | 15% |
| Student > Master | 8 | 11% |
| Student > Bachelor | 6 | 8% |
| Student > Doctoral Student | 4 | 6% |
| Other | 12 | 17% |
| Unknown | 18 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 35 | 49% |
| Biochemistry, Genetics and Molecular Biology | 12 | 17% |
| Chemical Engineering | 1 | 1% |
| Environmental Science | 1 | 1% |
| Computer Science | 1 | 1% |
| Other | 1 | 1% |
| Unknown | 20 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 July 2015.
All research outputs
#20,236,620
of 22,763,032 outputs
Outputs from BMC Plant Biology
#2,504
of 3,236 outputs
Outputs of similar age
#188,345
of 225,899 outputs
Outputs of similar age from BMC Plant Biology
#51
of 63 outputs
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