Title |
Estimating overannotation across prokaryotic genomes using BLAST+, UBLAST, LAST and BLAT
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Published in |
BMC Research Notes, September 2014
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DOI | 10.1186/1756-0500-7-651 |
Pubmed ID | |
Authors |
Gabriel Moreno-Hagelsieb, Brigitte Hudy-Yuffa |
Abstract |
As the number of genomes in public databases increases, it becomes more important to be able to quickly choose the best annotated genomes for further analyses in comparative genomics and evolution. A proxy to annotation quality is the estimation of overannotation by comparing annotated coding genes against the SwissProt database. NCBI's BLAST (BLAST+) is the common software of choice to compare these sequences. Newer programs that run in a fraction of the time as BLAST+ might miss matches that BLAST+ would find. However, the results might still be useful to calculate overannotation. We thus decided to compare the overannotation estimates yielded using three such programs, UBLAST, LAST and the Blast-Like Alignment Tool (BLAT), and to test non-redundant versions of the SwissProt database to reduce the number of comparisons necessary. |
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