Title |
Identification and characterization of microRNAs related to salt stress in broccoli, using high-throughput sequencing and bioinformatics analysis
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Published in |
BMC Plant Biology, September 2014
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DOI | 10.1186/s12870-014-0226-2 |
Pubmed ID | |
Authors |
Yunhong Tian, Yunming Tian, Xiaojun Luo, Tao Zhou, Zuoping Huang, Ying Liu, Yihan Qiu, Bing Hou, Dan Sun, Hongyu Deng, Shen Qian, Kaitai Yao |
Abstract |
MicroRNAs (miRNAs) are a new class of endogenous regulators of a broad range of physiological processes, which act by regulating gene expression post-transcriptionally. The brassica vegetable, broccoli (Brassica oleracea var. italica), is very popular with a wide range of consumers, but environmental stresses such as salinity are a problem worldwide in restricting its growth and yield. Little is known about the role of miRNAs in the response of broccoli to salt stress. In this study, broccoli subjected to salt stress and broccoli grown under control conditions were analyzed by high-throughput sequencing. Differential miRNA expression was confirmed by real-time reverse transcription polymerase chain reaction (RT-PCR). The prediction of miRNA targets was undertaken using the Kyoto Encyclopedia of Genes and Genomes (KEGG) Orthology (KO) database and Gene Ontology (GO)-enrichment analyses. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Mexico | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Practitioners (doctors, other healthcare professionals) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Iran, Islamic Republic of | 1 | 3% |
Unknown | 33 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 9 | 26% |
Student > Master | 8 | 24% |
Researcher | 8 | 24% |
Lecturer | 2 | 6% |
Professor | 1 | 3% |
Other | 3 | 9% |
Unknown | 3 | 9% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 18 | 53% |
Biochemistry, Genetics and Molecular Biology | 8 | 24% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 6% |
Earth and Planetary Sciences | 2 | 6% |
Environmental Science | 1 | 3% |
Other | 0 | 0% |
Unknown | 3 | 9% |