Title |
High-frequency aberrantly methylated targets in pancreatic adenocarcinoma identified via global DNA methylation analysis using methylCap-seq
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Published in |
Clinical Epigenetics, September 2014
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DOI | 10.1186/1868-7083-6-18 |
Pubmed ID | |
Authors |
Yangxing Zhao, Jinfeng Sun, Hongyu Zhang, Shicheng Guo, Jun Gu, Wei Wang, Ning Tang, Xiaoyu Zhou, Jian Yu |
Abstract |
Extensive reprogramming and dysregulation of DNA methylation is an important characteristic of pancreatic cancer (PC). Our study aimed to characterize the genomic methylation patterns in various genomic contexts of PC. The methyl capture sequencing (methylCap-seq) method was used to map differently methylated regions (DMRs) in pooled samples from ten PC tissues and ten adjacent non-tumor (PN) tissues. A selection of DMRs was validated in an independent set of PC and PN samples using methylation-specific PCR (MSP), bisulfite sequencing PCR (BSP), and methylation sensitive restriction enzyme-based qPCR (MSRE-qPCR). The mRNA and expressed sequence tag (EST) expression of the corresponding genes was investigated using RT-qPCR. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Germany | 2 | 33% |
United Kingdom | 2 | 33% |
United States | 1 | 17% |
Unknown | 1 | 17% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 4 | 67% |
Practitioners (doctors, other healthcare professionals) | 1 | 17% |
Members of the public | 1 | 17% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Netherlands | 1 | 5% |
United States | 1 | 5% |
Unknown | 18 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 35% |
Student > Ph. D. Student | 3 | 15% |
Lecturer | 2 | 10% |
Professor > Associate Professor | 2 | 10% |
Student > Master | 2 | 10% |
Other | 1 | 5% |
Unknown | 3 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 7 | 35% |
Agricultural and Biological Sciences | 5 | 25% |
Biochemistry, Genetics and Molecular Biology | 3 | 15% |
Social Sciences | 1 | 5% |
Unknown | 4 | 20% |