Title |
A rapid live-cell ELISA for characterizing antibodies against cell surface antigens of Chlamydomonas reinhardtii and its use in isolating algae from natural environments with related cell wall components
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Published in |
BMC Plant Biology, September 2014
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DOI | 10.1186/s12870-014-0244-0 |
Pubmed ID | |
Authors |
Wenzhi Jiang, Sarah Cossey, Julian N Rosenberg, George A Oyler, Bradley JSC Olson, Donald P Weeks |
Abstract |
Cell walls are essential for most bacteria, archaea, fungi, algae and land plants to provide shape, structural integrity and protection from numerous biotic and abiotic environmental factors. In the case of eukaryotic algae, relatively little is known of the composition, structure or mechanisms of assembly of cell walls in individual species or between species and how these differences enable algae to inhabit a great diversity of environments. In this paper we describe the use of camelid antibody fragments (VHHs) and a streamlined ELISA assay as powerful new tools for obtaining mono-specific reagents for detecting individual algal cell wall components and for isolating algae that share a particular cell surface component. |
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Mendeley readers
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