Title |
Elucidation of the outer membrane proteome of Salmonella enterica serovar Typhimurium utilising a lipid-based protein immobilization technique
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Published in |
BMC Microbiology, February 2010
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DOI | 10.1186/1471-2180-10-44 |
Pubmed ID | |
Authors |
Darren Chooneea, Roger Karlsson, Vesela Encheva, Cath Arnold, Hazel Appleton, Haroun Shah |
Abstract |
Salmonella enterica serovar Typhimurium (S. Typhimurium) is a major cause of human gastroenteritis worldwide. The outer membrane proteins expressed by S. Typhimurium mediate the process of adhesion and internalisation within the intestinal epithelium of the host thus influencing the progression of disease. Since the outer membrane proteins are surface-exposed, they provide attractive targets for the development of improved antimicrobial agents and vaccines. Various techniques have been developed for their characterisation, but issues such as carryover of cytosolic proteins still remain a problem. In this study we attempted to characterise the surface proteome of S. Typhimurium using Lipid-based Protein Immobilisation technology in the form of LPI FlowCells. No detergents are required and no sample clean up is needed prior to downstream analysis. The immobilised proteins can be digested with proteases in multiple steps to increase sequence coverage, and the peptides eluted can be characterised directly by liquid chromatography - tandem mass spectrometry (LC-MS/MS) and identified from mass spectral database searches. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Spain | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Scientists | 2 | 67% |
Members of the public | 1 | 33% |
Mendeley readers
Geographical breakdown
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United States | 2 | 3% |
Uruguay | 1 | 2% |
Unknown | 63 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 16 | 24% |
Professor > Associate Professor | 9 | 14% |
Student > Ph. D. Student | 8 | 12% |
Student > Master | 7 | 11% |
Student > Bachelor | 6 | 9% |
Other | 10 | 15% |
Unknown | 10 | 15% |
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Agricultural and Biological Sciences | 28 | 42% |
Biochemistry, Genetics and Molecular Biology | 6 | 9% |
Medicine and Dentistry | 4 | 6% |
Immunology and Microbiology | 4 | 6% |
Environmental Science | 3 | 5% |
Other | 9 | 14% |
Unknown | 12 | 18% |