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Complete genome sequence of Citrobacter werkmanii strain BF-6 isolated from industrial putrefaction

Overview of attention for article published in BMC Genomics, October 2017
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Title
Complete genome sequence of Citrobacter werkmanii strain BF-6 isolated from industrial putrefaction
Published in
BMC Genomics, October 2017
DOI 10.1186/s12864-017-4157-9
Pubmed ID
Authors

Gang Zhou, Hong Peng, Ying-si Wang, Xiao-mo Huang, Xiao-bao Xie, Qing-shan Shi

Abstract

In our previous study, Citrobacter werkmanii BF-6 was isolated from an industrial spoilage sample and demonstrated an excellent ability to form biofilms, which could be affected by various environmental factors. However, the genome sequence of this organism has not been reported so far. We report the complete genome sequence of C. werkmanii BF-6 together with the description of the genome features and its annotation. The size of the complete chromosome is 4,929,789 bp with an average coverage of 137×. The chromosome exhibits an average G + C content of 52.0%, and encodes 4570 protein coding genes, 84 tRNA genes, 25 rRNA operons, 3 microsatellite sequences and 34 minisatellite sequences. A previously unknown circular plasmid designated as pCW001 was also found with a length of 212,549 bp and a G + C content of 48.2%. 73.5%, 75.6% and 92.6% of the protein coding genes could be assigned to GO Ontology, KEGG Pathway, and COG (Clusters of Orthologous Groups) categories respectively. C. werkmanii BF-6 and C. werkmanii NRBC 105721 exhibited the closest evolutionary relationships based on 16S ribosomal RNA and core-pan genome assay. Furthermore, C. werkmanii BF-6 exhibits typical bacterial biofilm formation and development. In the RT-PCR experiments, we found that a great number of biofilm related genes, such as bsmA, bssR, bssS, hmsP, tabA, csgA, csgB, csgC, csgD, csgE, and csgG, were involved in C. werkmanii BF-6 biofilm formation. This is the first complete genome of C. werkmanii. Our work highlights the potential genetic mechanisms involved in biofilm formation and paves a way for further application of C. werkmanii in biofilms research.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 4 31%
Professor 2 15%
Student > Doctoral Student 2 15%
Student > Ph. D. Student 2 15%
Lecturer 1 8%
Other 1 8%
Unknown 1 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 31%
Immunology and Microbiology 3 23%
Environmental Science 1 8%
Chemical Engineering 1 8%
Agricultural and Biological Sciences 1 8%
Other 1 8%
Unknown 2 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 October 2017.
All research outputs
#17,917,778
of 23,005,189 outputs
Outputs from BMC Genomics
#7,612
of 10,692 outputs
Outputs of similar age
#232,082
of 324,392 outputs
Outputs of similar age from BMC Genomics
#126
of 196 outputs
Altmetric has tracked 23,005,189 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 10,692 research outputs from this source. They receive a mean Attention Score of 4.7. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
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We're also able to compare this research output to 196 others from the same source and published within six weeks on either side of this one. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.