Title |
Stage-specific differential gene expression in Leishmania infantum: from the foregut of Phlebotomus perniciosus to the human phagocyte
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Published in |
BMC Genomics, October 2014
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DOI | 10.1186/1471-2164-15-849 |
Pubmed ID | |
Authors |
Pedro J Alcolea, Ana Alonso, Manuel J Gómez, Marina Postigo, Ricardo Molina, Maribel Jiménez, Vicente Larraga |
Abstract |
Leishmania infantum is the etiological agent of zoonotical visceral leishmaniasis in the Mediterranean basin. A recent outbreak in humans has been recently reported in central Spain. Leishmania spp. parasites are transmitted to the mammalian host by the bite of sand flies. The primary vector of L. infantum in Spain is Phlebotomus perniciosus. For decades, research on these parasites has involved the axenic culture model of the promastigote stage including gene expression profiling studies performed in the post-genome era. Unlike the controversial axenic culturing of amastigotes, promastigote cultures are generally accepted and used, although with the precaution of avoiding excessive culture passage.The primary objective of this differentiation study is to compare the gene expression profiles of promastigotes isolated from the foregut of the sand fly and amastigotes. For this purpose, P. perniciosus sand flies were infected with L. infantum and differentiated promastigotes were extracted by dissection of the foreguts. Shotgun DNA microarray hybridization analyses allowed for transcriptome comparison of these promastigotes with amastigotes obtained by infection of the U937 cell line. The results have been compared with those described in published expression analyses using axenic promastigotes. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 1% |
Spain | 1 | 1% |
United States | 1 | 1% |
Brazil | 1 | 1% |
Unknown | 88 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 29 | 32% |
Researcher | 14 | 15% |
Student > Bachelor | 10 | 11% |
Student > Master | 9 | 10% |
Student > Doctoral Student | 7 | 8% |
Other | 14 | 15% |
Unknown | 9 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 24 | 26% |
Biochemistry, Genetics and Molecular Biology | 23 | 25% |
Immunology and Microbiology | 11 | 12% |
Medicine and Dentistry | 8 | 9% |
Veterinary Science and Veterinary Medicine | 3 | 3% |
Other | 9 | 10% |
Unknown | 14 | 15% |