Title |
Profiling of the embryonic Atlantic halibut (Hippoglossus hippoglossus L.) transcriptome reveals maternal transcripts as potential markers of embryo quality
|
---|---|
Published in |
BMC Genomics, September 2014
|
DOI | 10.1186/1471-2164-15-829 |
Pubmed ID | |
Authors |
Maren Mommens, Jorge MO Fernandes, Knut Erik Tollefsen, Ian A Johnston, Igor Babiak |
Abstract |
Commercial Atlantic halibut (Hippoglossus hippoglossus) farming is restricted by variable oocyte quality, slow growth, and early maturation of male fish. Maternally transferred components regulate early developmental processes; therefore, they have an effect on the future viability of the embryo. Using a newly developed Agilent 10 k custom-made oligonucleotide array, we profiled components of the transcriptome involved in immune defence as well as germline and muscle development during early developmental stages: 8-cell embryos (8CS), germ ring stage (GR), 10-somite stage (10SS), and hatched embryos (HT). In addition, we identified differentially expressed transcripts in low (<=9 +/- 3% hatching) and high (>=86 +/- 3[degree sign]% hatching) quality eggs at 8CS to identify potential maternal markers for embryo quality. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 38 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 26% |
Student > Master | 7 | 18% |
Researcher | 7 | 18% |
Professor | 3 | 8% |
Student > Doctoral Student | 2 | 5% |
Other | 5 | 13% |
Unknown | 4 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 22 | 58% |
Biochemistry, Genetics and Molecular Biology | 4 | 11% |
Unspecified | 2 | 5% |
Veterinary Science and Veterinary Medicine | 1 | 3% |
Environmental Science | 1 | 3% |
Other | 2 | 5% |
Unknown | 6 | 16% |