Title |
De novo assembly and characterization of the transcriptome of the toxic dinoflagellate Karenia brevis
|
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Published in |
BMC Genomics, October 2014
|
DOI | 10.1186/1471-2164-15-888 |
Pubmed ID | |
Authors |
Darcie E Ryan, Alan E Pepper, Lisa Campbell |
Abstract |
Karenia brevis is a harmful algal species that blooms in the Gulf of Mexico and produces brevetoxins that cause neurotoxic shellfish poisoning. Elevated brevetoxin levels in K. brevis cells have been measured during laboratory hypo-osmotic stress treatments. To investigate mechanisms underlying K. brevis osmoacclimation and osmoregulation and establish a valuable resource for gene discovery, we assembled reference transcriptomes for three clones: Wilson-CCFWC268, SP3, and SP1 (a low-toxin producing variant). K. brevis transcriptomes were annotated with gene ontology terms and searched for putative transmembrane proteins that may elucidate cellular responses to hypo-osmotic stress. An analysis of single nucleotide polymorphisms among clones was used to characterize genetic divergence. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 2 | 33% |
India | 2 | 33% |
France | 1 | 17% |
Unknown | 1 | 17% |
Demographic breakdown
Type | Count | As % |
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Scientists | 5 | 83% |
Members of the public | 1 | 17% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Japan | 1 | 2% |
United Kingdom | 1 | 2% |
Russia | 1 | 2% |
Unknown | 53 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 17 | 30% |
Researcher | 12 | 21% |
Student > Master | 6 | 11% |
Professor > Associate Professor | 5 | 9% |
Student > Doctoral Student | 4 | 7% |
Other | 8 | 14% |
Unknown | 4 | 7% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 13 | 23% |
Environmental Science | 5 | 9% |
Veterinary Science and Veterinary Medicine | 1 | 2% |
Computer Science | 1 | 2% |
Other | 2 | 4% |
Unknown | 7 | 13% |