Title |
Effect of holding equine oocytes in meiosis inhibitor-free medium before in vitro maturation and of holding temperature on meiotic suppression and mitochondrial energy/redox potential
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Published in |
Reproductive Biology and Endocrinology, October 2014
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DOI | 10.1186/1477-7827-12-99 |
Pubmed ID | |
Authors |
Nicola A Martino, Maria E Dell’Aquila, Manuel Filioli Uranio, Lucia Rutigliano, Michele Nicassio, Giovanni M Lacalandra, Katrin Hinrichs |
Abstract |
Evaluation of mitochondria function offers an alternative to evaluate embryo development for assessment of oocyte viability, but little information is available on the relationship between mitochondria and chromatin status in equine oocytes. We evaluated these parameters in immature equine oocytes either fixed immediately (IMM) or held overnight in an Earle's/Hank's' M199-based medium in the absence of meiotic inhibitors (EH treatment), and in mature oocytes. We hypothesized that EH holding may affect mitochondria function and that holding temperature may affect the efficiency of meiotic suppression. |
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France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 38 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 8 | 21% |
Researcher | 5 | 13% |
Student > Ph. D. Student | 5 | 13% |
Professor | 3 | 8% |
Student > Postgraduate | 3 | 8% |
Other | 7 | 18% |
Unknown | 7 | 18% |
Readers by discipline | Count | As % |
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Veterinary Science and Veterinary Medicine | 12 | 32% |
Agricultural and Biological Sciences | 12 | 32% |
Medicine and Dentistry | 5 | 13% |
Biochemistry, Genetics and Molecular Biology | 2 | 5% |
Unknown | 7 | 18% |