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A novel method to follow meiotic progression in Arabidopsis using confocal microscopy and 5-ethynyl-2′-deoxyuridine labeling

Overview of attention for article published in Plant Methods, October 2014
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Title
A novel method to follow meiotic progression in Arabidopsis using confocal microscopy and 5-ethynyl-2′-deoxyuridine labeling
Published in
Plant Methods, October 2014
DOI 10.1186/1746-4811-10-33
Pubmed ID
Authors

Patti E Stronghill, Wajma Azimi, Clare A Hasenkampf

Abstract

Meiosis progression in the more recent past has been investigated using 5-bromo-2'-deoxyuridine (BrdU) uptake by S-phase meiocytes undergoing DNA replication. BrdU uptake is detected by reaction with BrdU antibody followed by epifluorescent microscopy examination of chromosome spreads and/or squashes. We here report using confocal microscopic examination of intact meiocytes in conjunction with the new thymidine analog 5-ethynyl-2'-deoxyuridine (EdU). The simplicity of the EdU detection coupled with confocal examination of anthers provides a more exact temporal description of meiotic prophase I progression in Arabidopsis and opens up the possibility of examining the coordination of microsporocyte development with the other tissues of the anther.

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X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 50 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 22%
Researcher 9 18%
Student > Master 7 14%
Student > Bachelor 3 6%
Student > Doctoral Student 3 6%
Other 8 16%
Unknown 9 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 27 54%
Biochemistry, Genetics and Molecular Biology 12 24%
Unspecified 1 2%
Unknown 10 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 October 2014.
All research outputs
#14,787,304
of 22,766,595 outputs
Outputs from Plant Methods
#777
of 1,080 outputs
Outputs of similar age
#141,244
of 255,778 outputs
Outputs of similar age from Plant Methods
#7
of 10 outputs
Altmetric has tracked 22,766,595 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,080 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 255,778 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.