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MultiBac: from protein complex structures to synthetic viral nanosystems

Overview of attention for article published in BMC Biology, October 2017
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Title
MultiBac: from protein complex structures to synthetic viral nanosystems
Published in
BMC Biology, October 2017
DOI 10.1186/s12915-017-0447-6
Pubmed ID
Authors

Martin Pelosse, Hannah Crocker, Barbara Gorda, Paul Lemaire, Jens Rauch, Imre Berger

Abstract

The MultiBac baculovirus/insect cell expression vector system was conceived as a user-friendly, modular tool-kit for producing multiprotein complexes for structural biology applications. MultiBac has allowed the structure and function of many molecular machines to be elucidated, including previously inaccessible high-value drug targets. More recently, MultiBac developments have shifted to customized baculoviral genomes that are tailored for a range of applications, including synthesizing artificial proteins by genetic code expansion. We review some of these developments, including the ongoing rewiring of the MultiBac system for mammalian applications, notably CRISPR/Cas9-mediated gene editing.

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The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 94 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 94 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 31 33%
Student > Master 14 15%
Student > Ph. D. Student 12 13%
Student > Bachelor 6 6%
Student > Doctoral Student 4 4%
Other 5 5%
Unknown 22 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 45 48%
Agricultural and Biological Sciences 14 15%
Chemistry 4 4%
Immunology and Microbiology 3 3%
Engineering 2 2%
Other 3 3%
Unknown 23 24%