Title |
MultiBac: from protein complex structures to synthetic viral nanosystems
|
---|---|
Published in |
BMC Biology, October 2017
|
DOI | 10.1186/s12915-017-0447-6 |
Pubmed ID | |
Authors |
Martin Pelosse, Hannah Crocker, Barbara Gorda, Paul Lemaire, Jens Rauch, Imre Berger |
Abstract |
The MultiBac baculovirus/insect cell expression vector system was conceived as a user-friendly, modular tool-kit for producing multiprotein complexes for structural biology applications. MultiBac has allowed the structure and function of many molecular machines to be elucidated, including previously inaccessible high-value drug targets. More recently, MultiBac developments have shifted to customized baculoviral genomes that are tailored for a range of applications, including synthesizing artificial proteins by genetic code expansion. We review some of these developments, including the ongoing rewiring of the MultiBac system for mammalian applications, notably CRISPR/Cas9-mediated gene editing. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Ireland | 1 | 25% |
Unknown | 3 | 75% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 50% |
Scientists | 1 | 25% |
Science communicators (journalists, bloggers, editors) | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 94 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 31 | 33% |
Student > Master | 14 | 15% |
Student > Ph. D. Student | 12 | 13% |
Student > Bachelor | 6 | 6% |
Student > Doctoral Student | 4 | 4% |
Other | 5 | 5% |
Unknown | 22 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 45 | 48% |
Agricultural and Biological Sciences | 14 | 15% |
Chemistry | 4 | 4% |
Immunology and Microbiology | 3 | 3% |
Engineering | 2 | 2% |
Other | 3 | 3% |
Unknown | 23 | 24% |