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Comparison of intracellular and secretion-based strategies for production of human α-galactosidase A in the filamentous fungus Trichoderma reesei

Overview of attention for article published in BMC Biotechnology, October 2014
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (81st percentile)

Mentioned by

news
1 news outlet

Citations

dimensions_citation
9 Dimensions

Readers on

mendeley
35 Mendeley
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Title
Comparison of intracellular and secretion-based strategies for production of human α-galactosidase A in the filamentous fungus Trichoderma reesei
Published in
BMC Biotechnology, October 2014
DOI 10.1186/s12896-014-0091-y
Pubmed ID
Authors

Wesley Smith, Jussi Jäntti, Merja Oja, Markku Saloheimo

Abstract

Trichoderma reesei is known as a good producer of industrial proteins but has hitherto been less successful in the production of therapeutic proteins. In order to elucidate the bottlenecks of heterologous protein production, human α-galactosidase A (GLA) was chosen as a model therapeutic protein. Fusion partners were designed to compare the effects of secretion using a cellobiohydrolase I (CBHI) carrier and intracellular production using a gamma zein peptide from maize (ZERA) which accumulates inside the endoplasmic reticulum (ER). The two strategies were compared on the basis of expression levels, purification performance, enzymatic activity, bioreactor cultivations, and transcriptional profiling.

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 3%
France 1 3%
Unknown 33 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 17%
Researcher 6 17%
Student > Master 5 14%
Student > Bachelor 3 9%
Student > Doctoral Student 2 6%
Other 6 17%
Unknown 7 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 29%
Biochemistry, Genetics and Molecular Biology 9 26%
Engineering 3 9%
Computer Science 2 6%
Medicine and Dentistry 1 3%
Other 2 6%
Unknown 8 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 October 2014.
All research outputs
#1,304,807
of 8,702,492 outputs
Outputs from BMC Biotechnology
#113
of 502 outputs
Outputs of similar age
#34,901
of 203,809 outputs
Outputs of similar age from BMC Biotechnology
#14
of 22 outputs
Altmetric has tracked 8,702,492 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 502 research outputs from this source. They receive a mean Attention Score of 4.5. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 203,809 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.