Title |
The oxygen-independent metabolism of cyclic monoterpenes in Castellaniella defragrans 65Phen
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Published in |
BMC Microbiology, June 2014
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DOI | 10.1186/1471-2180-14-164 |
Pubmed ID | |
Authors |
Jan Petasch, Eva-Maria Disch, Stephanie Markert, Dörte Becher, Thomas Schweder, Bruno Hüttel, Richard Reinhardt, Jens Harder |
Abstract |
The facultatively anaerobic betaproteobacterium Castellaniella defragrans 65Phen utilizes acyclic, monocyclic and bicyclic monoterpenes as sole carbon source under oxic as well as anoxic conditions. A biotransformation pathway of the acyclic β-myrcene required linalool dehydratase-isomerase as initial enzyme acting on the hydrocarbon. An in-frame deletion mutant did not use myrcene, but was able to grow on monocyclic monoterpenes. The genome sequence and a comparative proteome analysis together with a random transposon mutagenesis were conducted to identify genes involved in the monocyclic monoterpene metabolism. Metabolites accumulating in cultures of transposon and in-frame deletion mutants disclosed the degradation pathway. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 61 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 12 | 20% |
Researcher | 12 | 20% |
Student > Master | 7 | 11% |
Student > Bachelor | 6 | 10% |
Student > Postgraduate | 4 | 7% |
Other | 9 | 15% |
Unknown | 11 | 18% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 26 | 43% |
Biochemistry, Genetics and Molecular Biology | 9 | 15% |
Chemistry | 5 | 8% |
Environmental Science | 3 | 5% |
Medicine and Dentistry | 2 | 3% |
Other | 3 | 5% |
Unknown | 13 | 21% |