Title |
Expression of endoglucanases in Pichia pastoris under control of the GAP promoter
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Published in |
Microbial Cell Factories, April 2014
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DOI | 10.1186/1475-2859-13-57 |
Pubmed ID | |
Authors |
Anikó Várnai, Campbell Tang, Oskar Bengtsson, Andrew Atterton, Geir Mathiesen, Vincent GH Eijsink |
Abstract |
Plant-derived biomass is a potential alternative to fossil feedstocks for a greener economy. Enzymatic saccharification of biomass has been studied extensively and endoglucanases have been found to be a prerequisite for quick initial liquefaction of biomass under industrial conditions. Pichia pastoris, widely used for heterologous protein expression, can be utilized for fungal endoglucanase production. The recently marketed PichiaPink™ expression system allows for rapid clone selection, and employs the methanol inducible AOX1 promoter to ensure high protein expression levels. However, methanol is toxic and poses a fire hazard, issues which become more significant at an industrial scale. It is possible to eliminate these risks and still maintain high productivity by switching to the constitutive GAP promoter. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | <1% |
Austria | 1 | <1% |
Unknown | 167 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 38 | 22% |
Researcher | 31 | 18% |
Student > Master | 29 | 17% |
Student > Bachelor | 14 | 8% |
Student > Postgraduate | 6 | 4% |
Other | 17 | 10% |
Unknown | 34 | 20% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 55 | 33% |
Biochemistry, Genetics and Molecular Biology | 45 | 27% |
Engineering | 6 | 4% |
Chemistry | 5 | 3% |
Environmental Science | 4 | 2% |
Other | 20 | 12% |
Unknown | 34 | 20% |