Title |
Probiotics and virulent human rotavirus modulate the transplanted human gut microbiota in gnotobiotic pigs
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Published in |
Gut Pathogens, September 2014
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DOI | 10.1186/s13099-014-0039-8 |
Pubmed ID | |
Authors |
Husen Zhang, Haifeng Wang, Megan Shepherd, Ke Wen, Guohua Li, Xingdong Yang, Jacob Kocher, Ernawati Giri-Rachman, Allan Dickerman, Robert Settlage, Lijuan Yuan |
Abstract |
We generated a neonatal pig model with human infant gut microbiota (HGM) to study the effect of a probiotic on the composition of the transplanted microbiota following rotavirus vaccination and challenge. All the HGM-transplanted pigs received two doses of an oral attenuated rotavirus vaccine. The gut microbiota of vaccinated pigs were investigated for effects of Lactobacillus rhamnosus GG (LGG) supplement and homotypic virulent human rotavirus (HRV) challenge. High-throughput sequencing of V4 region of 16S rRNA genes demonstrated that HGM-transplanted pigs carried microbiota similar to that of the C-section delivered baby. Firmicutes and Proteobacteria represented over 98% of total bacteria in the human donor and the recipient pigs. HRV challenge caused a phylum-level shift from Firmicutes to Proteobacteria. LGG supplement prevented the changes in microbial communities caused by HRV challenge. In particular, members of Enterococcus in LGG-supplemented pigs were kept at the baseline level, while they were enriched in HRV challenged pigs. Taken together, our results suggested that HGM pigs are valuable for testing the microbiota's response to probiotic interventions for treating infantile HRV infection. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 60 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 13 | 22% |
Student > Bachelor | 8 | 13% |
Student > Ph. D. Student | 7 | 12% |
Researcher | 6 | 10% |
Other | 3 | 5% |
Other | 7 | 12% |
Unknown | 16 | 27% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 14 | 23% |
Biochemistry, Genetics and Molecular Biology | 7 | 12% |
Medicine and Dentistry | 6 | 10% |
Immunology and Microbiology | 5 | 8% |
Environmental Science | 3 | 5% |
Other | 10 | 17% |
Unknown | 15 | 25% |