Title |
D2-like receptor activation does not initiate a brain docosahexaenoic acid signal in unanesthetized rats
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Published in |
BMC Neuroscience, October 2014
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DOI | 10.1186/1471-2202-15-113 |
Pubmed ID | |
Authors |
Ameer Y Taha, Lisa Chang, Mei Chen, Stanley I Rapoport, Epolia Ramadan |
Abstract |
The polyunsaturated fatty acid, docosahexaenoic acid (DHA), participates in neurotransmission involving activation of calcium-independent phospholipase A2 (iPLA2), which is coupled to muscarinic, cholinergic and serotonergic neuroreceptors. Drug induced activation of iPLA2 can be measured in vivo with quantitative autoradiography using 14C-DHA as a probe. The present study used this approach to address whether a DHA signal is produced following dompaminergic (D)2-like receptor activation with quinpirole in rat brain. Unanesthetized rats were infused intravenously with 14C-DHA one minute after saline or quinpirole infusion, and serial blood samples were collected over a 20-minute period to obtain plasma. The animals were euthanized with sodium pentobarbital and their brains excised, coronally dissected and subjected to quantitative autoradiography to derive the regional incorporation coefficient, k*, a marker of DHA signaling. Plasma labeled and unlabeled unesterified DHA concentrations were measured. |
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