Title |
Filtering of MS/MS data for peptide identification
|
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Published in |
BMC Genomics, November 2013
|
DOI | 10.1186/1471-2164-14-s7-s2 |
Pubmed ID | |
Authors |
Jason Gallia, Katelyn Lavrich, Anna Tan-Wilson, Patrick H Madden |
Abstract |
The identification of proteins based on analysis of tandem mass spectrometry (MS/MS) data is a valuable tool that is not fully realized because of the difficulty in carrying out automated analysis of large numbers of spectra. MS/MS spectra consist of peaks that represent each peptide fragment, usually b and y ions, with experimentally determined mass to charge ratios. Whether the strategy employed is database matching or De Novo sequencing, a major obstacle is distinguishing signal from noise. Improved ability to distinguish signal peaks of low intensity from background noise increases the likelihood of correctly identifying the peptide, as valuable information is preserved while extraneous information is not left to mislead. |
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