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Mendeley readers
| Title |
Identification of a virulence-related surface protein XF in piscine Streptococcus agalactiaeby pre-absorbed immunoproteomics
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|---|---|
| Published in |
BMC Veterinary Research, October 2014
|
| DOI | 10.1186/s12917-014-0259-7 |
| Pubmed ID | |
| Authors |
Guangjin Liu, Wei Zhang, Yongjie Liu, Huochun Yao, Chengping Lu, Pao Xu |
| Abstract |
BackgroundSince 2009, large-scale Streptococcus agalactiae infections have broken out in cultured tilapia farms in China, resulting in considerable economic losses. Screening of the surface proteins is required to identify virulence factors or protective antigens involved in piscine S.agalactiae infections in tilapia. Pre-absorbed immunoproteomics method (PAIM) is a useful method previously established in our laboratory for identifying bacterial surface proteins.ResultsA serine-rich repeat protein family 1 (Srr-1), designated XF, was identified by PAIM in piscine S. agalactiae isolate GD201008-001. To investigate the role of XF in the pathogenesis of piscine S. agalactiae, an isogenic xf mutant strain (¿xf) and a complemented strain (C¿xf) were successfully constructed. The ¿xf mutant and C¿xf showed no significant differences in growth characteristics and adherence to HEp-2 cells compared with the wild-type strain. However the 50% lethal dose of ¿xf was increased (4-fold) compared with that of the parental strain in a zebrafish infection model.ConclusionsThe findings demonstrated that XF is a virulence-related, highly immunoreactive surface protein and is involved in the pathogenicity of S. agalactiae infections in fish. |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 16% |
| Student > Doctoral Student | 2 | 11% |
| Other | 2 | 11% |
| Researcher | 2 | 11% |
| Student > Postgraduate | 2 | 11% |
| Other | 3 | 16% |
| Unknown | 5 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 3 | 16% |
| Agricultural and Biological Sciences | 3 | 16% |
| Environmental Science | 1 | 5% |
| Biochemistry, Genetics and Molecular Biology | 1 | 5% |
| Nursing and Health Professions | 1 | 5% |
| Other | 3 | 16% |
| Unknown | 7 | 37% |