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Heterologous complementation of a pyrF deletion in Caldicellulosiruptor hydrothermalisgenerates a new host for the analysis of biomass deconstruction

Overview of attention for article published in Biotechnology for Biofuels and Bioproducts, September 2014
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Title
Heterologous complementation of a pyrF deletion in Caldicellulosiruptor hydrothermalisgenerates a new host for the analysis of biomass deconstruction
Published in
Biotechnology for Biofuels and Bioproducts, September 2014
DOI 10.1186/s13068-014-0132-8
Pubmed ID
Authors

Joseph Groom, Daehwan Chung, Jenna Young, Janet Westpheling

Abstract

Members of the thermophilic, anaerobic Gram-positive bacterial genus Caldicellulosiruptor grow optimally at 65 to 78°C and degrade lignocellulosic biomass without conventional pretreatment. Decomposition of complex cell wall polysaccharides is a major bottleneck in the conversion of plant biomass to biofuels and chemicals, and conventional biomass pretreatment includes exposure to high temperatures, acids, or bases as well as enzymatic digestion. Members of this genus contain a variety of glycosyl hydrolases, pectinases, and xylanases, but the contribution of these individual enzymes to biomass deconstruction is largely unknown. C. hydrothermalis is of special interest because it is the least cellulolytic of all the Caldicellulosiruptor species so far characterized, making it an ideal naïve system to study key cellulolytic enzymes from these bacteria.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Indonesia 1 4%
Unknown 23 96%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 25%
Student > Ph. D. Student 5 21%
Researcher 4 17%
Other 3 13%
Professor 1 4%
Other 2 8%
Unknown 3 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 38%
Biochemistry, Genetics and Molecular Biology 6 25%
Engineering 3 13%
Chemical Engineering 2 8%
Unspecified 1 4%
Other 1 4%
Unknown 2 8%