Title |
Heterologous complementation of a pyrF deletion in Caldicellulosiruptor hydrothermalisgenerates a new host for the analysis of biomass deconstruction
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Published in |
Biotechnology for Biofuels and Bioproducts, September 2014
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DOI | 10.1186/s13068-014-0132-8 |
Pubmed ID | |
Authors |
Joseph Groom, Daehwan Chung, Jenna Young, Janet Westpheling |
Abstract |
Members of the thermophilic, anaerobic Gram-positive bacterial genus Caldicellulosiruptor grow optimally at 65 to 78°C and degrade lignocellulosic biomass without conventional pretreatment. Decomposition of complex cell wall polysaccharides is a major bottleneck in the conversion of plant biomass to biofuels and chemicals, and conventional biomass pretreatment includes exposure to high temperatures, acids, or bases as well as enzymatic digestion. Members of this genus contain a variety of glycosyl hydrolases, pectinases, and xylanases, but the contribution of these individual enzymes to biomass deconstruction is largely unknown. C. hydrothermalis is of special interest because it is the least cellulolytic of all the Caldicellulosiruptor species so far characterized, making it an ideal naïve system to study key cellulolytic enzymes from these bacteria. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Indonesia | 1 | 4% |
Unknown | 23 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 6 | 25% |
Student > Ph. D. Student | 5 | 21% |
Researcher | 4 | 17% |
Other | 3 | 13% |
Professor | 1 | 4% |
Other | 2 | 8% |
Unknown | 3 | 13% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 9 | 38% |
Biochemistry, Genetics and Molecular Biology | 6 | 25% |
Engineering | 3 | 13% |
Chemical Engineering | 2 | 8% |
Unspecified | 1 | 4% |
Other | 1 | 4% |
Unknown | 2 | 8% |