Title |
IS element IS16 as a molecular screening tool to identify hospital-associated strains of Enterococcus faecium
|
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Published in |
BMC Infectious Diseases, March 2011
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DOI | 10.1186/1471-2334-11-80 |
Pubmed ID | |
Authors |
Guido Werner, Carola Fleige, Uta Geringer, Willem van Schaik, Ingo Klare, Wolfgang Witte |
Abstract |
Hospital strains of Enterococcus faecium could be characterized and typed by various molecular methods (MLST, AFLP, MLVA) and allocated to a distinct clonal complex known as MLST CC17. However, these techniques are laborious, time-consuming and cost-intensive. Our aim was to identify hospital E. faecium strains and differentiate them from colonizing and animal variants by a simple, inexpensive and reliable PCR-based screening assay. We describe here performance and predictive value of a single PCR detecting the insertion element, IS16, to identify hospital E. faecium isolates within a collection of 260 strains of hospital, animal and human commensal origins. |
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Student > Ph. D. Student | 16 | 17% |
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Nursing and Health Professions | 2 | 2% |
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