Title |
An improved 2b-RAD approach (I2b-RAD) offering genotyping tested by a rice (Oryza sativa L.) F2 population
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Published in |
BMC Genomics, November 2014
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DOI | 10.1186/1471-2164-15-956 |
Pubmed ID | |
Authors |
Yu Guo, Hui Yuan, Dongming Fang, Lianbo Song, Yan Liu, Yong Liu, Lu Wu, Jianping Yu, Zichao Li, Xun Xu, Hongliang Zhang |
Abstract |
2b-RAD (type IIB endonucleases restriction-site associated DNA) approach was invented by Wang in 2012 and proven as a simple and flexible method for genome-wide genotyping. However, there is still plenty of room for improvement for the existent 2b-RAD approach. Firstly, it doesn't include the samples pooling in library preparation as other reduced representation libraries. Secondly, the information of 2b-RAD tags, such as tags numbers and distributions, in most of species are unknown. The purposes of the research are to improve a new 2b-RAD approach which possesses samples pooling, moreover to figure out the characteristic and application potentiality of 2b-RAD tags by bioinformatics analysis. |
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Geographical breakdown
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United Kingdom | 1 | 1% |
Spain | 1 | 1% |
United States | 1 | 1% |
Italy | 1 | 1% |
Unknown | 73 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 17 | 22% |
Researcher | 17 | 22% |
Student > Master | 8 | 10% |
Other | 6 | 8% |
Student > Doctoral Student | 5 | 6% |
Other | 12 | 16% |
Unknown | 12 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 39 | 51% |
Biochemistry, Genetics and Molecular Biology | 12 | 16% |
Environmental Science | 5 | 6% |
Medicine and Dentistry | 2 | 3% |
Unspecified | 1 | 1% |
Other | 2 | 3% |
Unknown | 16 | 21% |