Title |
Proteomic analysis of human placental syncytiotrophoblast microvesicles in preeclampsia
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Published in |
Clinical Proteomics, November 2014
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DOI | 10.1186/1559-0275-11-40 |
Pubmed ID | |
Authors |
Sonia Baig, Narasimhan Kothandaraman, Jayapal Manikandan, Li Rong, Kim Huey EE, Jeffrey Hill, Chin Wee Lai, Wan Yu Tan, Felicia Yeoh, Anita Kale, Lin Lin Su, Arijit Biswas, Sheila Vasoo, Mahesh Choolani |
Abstract |
Placental syncytiotrophoblast microvesicles (STBM) are shed into the maternal circulation during normal pregnancy. STBM circulate in significantly increased amounts in preeclampsia (PE) and are considered to be among contributors to the exaggerated proinflammatory, procoagulant state of PE. However, protein composition of STBM in normal pregnancy and PE remains unknown. We therefore sought to determine the protein components of STBM and whether STBM protein expressions differ in preeclamptic and normal pregnancies. Patients with PE (n = 3) and normal pregnant controls (n = 6) were recruited. STBM were prepared from placental explant culture supernatant. STBM proteins were analyzed by a combination of 1D Gel-LC-MS/MS. Protein expressions levels were quantified using spectral counts and validated by immunohistochemistry. |
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Demographic breakdown
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Scientists | 1 | 33% |
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Mendeley readers
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