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Mendeley readers
Attention Score in Context
| Title |
Development of an enzyme linked immunosorbent assay for detection of cyathane diterpenoids
|
|---|---|
| Published in |
BMC Biotechnology, November 2014
|
| DOI | 10.1186/s12896-014-0098-4 |
| Pubmed ID | |
| Authors |
Tian Shen, Lena M Hof, Heike Hausmann, Marc Stadler, Holger Zorn |
| Abstract |
BackgroundSo-called cyathane type diterpenoids are produced as secondary metabolites by basidiomycetes. Based on their antibacterial, fungicidal, and cytotoxic properties, cyathane type terpenoids represent interesting target compounds in fungal biotechnology.ResultsAn indirect competitive enzyme linked immunosorbent assay has been developed for detection of cyathane type diterpenoids. Rabbit polyclonal antibodies were raised against a mixture of striatal A and B conjugated to bovine serum albumin. The conditions for direct attachment of the hapten striatal B to a solid phase by passive adsorption were optimized. The cross reactivities of the striatals A, C and D, of the striatins A and B, and of the erinacines C and P to striatal B were determined. The validation study showed that the ELISA was precise and sensitive. The average IC50 of striatal B was 36.0 ng mL¿1 with an inter-assay coefficient of variation (CV) of 13.2% (n =5). Recoveries from striatal B spiked samples in the assay were in the range of 97.3 ¿ 125.9%. A good correlation between the striatal B concentration measured by the ELISA and by HPLC-DAD (y =1.1122x ¿ 0.1585, R2¿=¿0.9942) was obtained from linear regression analysis. The suitability of the ELISA for detection of cyathane type diterpenoids in submerged cultures and fruiting bodies of H. erinaceus was studied. It showed cross reactivity with supernatants from submerged cultures and extracts thereof, but did not show cross reactivity with extracts from fruiting bodies.ConclusionsThe developed method is appropriate for qualitative and quantitative detection of cyathane diterpenoids in complex mixtures. Due to its high sensitivity and specificity, it represents an ideal screening method for discovering new cyathane diterpenoids and new potential producers of them. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 5% |
| Unknown | 19 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 15% |
| Professor | 3 | 15% |
| Researcher | 3 | 15% |
| Student > Doctoral Student | 2 | 10% |
| Student > Ph. D. Student | 2 | 10% |
| Other | 4 | 20% |
| Unknown | 3 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 45% |
| Chemistry | 4 | 20% |
| Biochemistry, Genetics and Molecular Biology | 2 | 10% |
| Medicine and Dentistry | 1 | 5% |
| Unknown | 4 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 November 2014.
All research outputs
#4,167,468
of 22,771,140 outputs
Outputs from BMC Biotechnology
#213
of 935 outputs
Outputs of similar age
#60,009
of 362,492 outputs
Outputs of similar age from BMC Biotechnology
#19
of 28 outputs
Altmetric has tracked 22,771,140 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 935 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.7. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 362,492 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 28 others from the same source and published within six weeks on either side of this one. This one is in the 7th percentile – i.e., 7% of its contemporaries scored the same or lower than it.