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Leukocyte telomere length variation due to DNA extraction method

Overview of attention for article published in BMC Research Notes, December 2014
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Citations

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Title
Leukocyte telomere length variation due to DNA extraction method
Published in
BMC Research Notes, December 2014
DOI 10.1186/1756-0500-7-877
Pubmed ID
Authors

Joshua Denham, Francine Z Marques, Fadi J Charchar

Abstract

Telomere length is indicative of biological age. Shorter telomeres have been associated with several disease and health states. There are inconsistencies throughout the literature amongst relative telomere length measured by quantitative PCR (qPCR) and different extraction methods or kits used. We quantified whole-blood leukocyte telomere length using the telomere to single copy gene (T/S) ratio by qPCR in 20 young (18-25 yrs) men after extracting DNA using three common extraction methods: Lahiri and Nurnberger (high salt) method, PureLink Genomic DNA Mini kit (Life Technologies) and QiaAmp DNA Mini kit (Qiagen). Telomere length differences of DNA extracted from the three extraction methods was assessed by one-way analysis of variance (ANOVA).

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 2%
Unknown 51 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 12 23%
Student > Bachelor 10 19%
Researcher 9 17%
Student > Master 4 8%
Professor 3 6%
Other 6 12%
Unknown 8 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 19%
Agricultural and Biological Sciences 9 17%
Medicine and Dentistry 6 12%
Immunology and Microbiology 3 6%
Neuroscience 2 4%
Other 10 19%
Unknown 12 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 December 2014.
All research outputs
#15,311,799
of 22,772,779 outputs
Outputs from BMC Research Notes
#2,314
of 4,263 outputs
Outputs of similar age
#213,581
of 360,775 outputs
Outputs of similar age from BMC Research Notes
#42
of 94 outputs
Altmetric has tracked 22,772,779 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,263 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.5. This one is in the 33rd percentile – i.e., 33% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 360,775 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 94 others from the same source and published within six weeks on either side of this one. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.