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Leukocyte telomere length variation due to DNA extraction method

Overview of attention for article published in BMC Research Notes, December 2014
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1 tweeter

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38 Mendeley
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1 CiteULike
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Title
Leukocyte telomere length variation due to DNA extraction method
Published in
BMC Research Notes, December 2014
DOI 10.1186/1756-0500-7-877
Pubmed ID
Authors

Joshua Denham, Francine Z Marques, Fadi J Charchar

Abstract

Telomere length is indicative of biological age. Shorter telomeres have been associated with several disease and health states. There are inconsistencies throughout the literature amongst relative telomere length measured by quantitative PCR (qPCR) and different extraction methods or kits used. We quantified whole-blood leukocyte telomere length using the telomere to single copy gene (T/S) ratio by qPCR in 20 young (18-25 yrs) men after extracting DNA using three common extraction methods: Lahiri and Nurnberger (high salt) method, PureLink Genomic DNA Mini kit (Life Technologies) and QiaAmp DNA Mini kit (Qiagen). Telomere length differences of DNA extracted from the three extraction methods was assessed by one-way analysis of variance (ANOVA).

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 3%
Unknown 37 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 29%
Student > Bachelor 8 21%
Student > Master 4 11%
Researcher 4 11%
Professor 3 8%
Other 6 16%
Unknown 2 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 24%
Medicine and Dentistry 5 13%
Biochemistry, Genetics and Molecular Biology 4 11%
Environmental Science 2 5%
Neuroscience 2 5%
Other 10 26%
Unknown 6 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 December 2014.
All research outputs
#2,355,584
of 4,586,246 outputs
Outputs from BMC Research Notes
#670
of 1,400 outputs
Outputs of similar age
#74,456
of 147,758 outputs
Outputs of similar age from BMC Research Notes
#46
of 99 outputs
Altmetric has tracked 4,586,246 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,400 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 37th percentile – i.e., 37% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 147,758 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 99 others from the same source and published within six weeks on either side of this one. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.