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Attention Score in Context
| Title |
Orphan nuclear receptor Nur77 Inhibits Oxidized LDL-induced differentiation of RAW264.7 murine macrophage cell line into dendritic like cells
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|---|---|
| Published in |
BMC Immunology, November 2014
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| DOI | 10.1186/s12865-014-0054-z |
| Pubmed ID | |
| Authors |
Liu-hua Hu, Ying Yu, Shu-xuan Jin, Peng Nie, Zhao-hua Cai, Ming-li Cui, Shi-qun Sun, Hua Xiao, Qin Shao, Ling-hong Shen, Ben He |
| Abstract |
BackgroundNur77 is an orphan nuclear receptor expressed in human atheroma. In vascular cells in vitro, Nur77 expression is induced by pro-inflammatory factors, such as oxidized LDL (oxLDL).MethodsWe analyze the role of Nur77 in the oxLDL-induced differentiation of macrophages into dendritic cells (DC). The murine RAW264.7 macrophage cell line was stably transfected with expression plasmids encoding either GFP or GFP fusions with either full-length Nur77 (GFP-Nur77), Nur77 lacking the DNA binding domain (GFP-Nur77-¿DBD) or Nur77 lacking the transactivation domain (GFP-Nur77-¿TAD).ResultsGFP-Nur77 overexpression significantly suppressed the effect of oxLDL treatment on DC morphologic changes, expression of DC maturation markers, endocytic activity, allogeneic activation of T cell proliferation, and the activity and secretion of pro-inflammatory cytokines. Analysis of GFP-Nur77-¿TAD and GFP-Nur77-¿DBD indicated that the Nur77 DNA binding and transactivation domains were both required for this effect. GFP-Nur77-¿DBD consistently had the opposite effect to GFP-Nur77, increasing DC-type differentiation in all assays. Interestingly, GFP-Nur77-¿DBD protein was cytosolic, whereas GFP-Nur77 and GFP-Nur77-¿TAD were both nuclear.ConclusionsThese data show that GFP-Nur77 inhibited differentiation of oxLDL-treated macrophages into DC. The effects of Nur77 on the macrophage phenotype may involve changes in its subcellular distribution. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 25% |
| Student > Bachelor | 2 | 25% |
| Other | 1 | 13% |
| Unknown | 3 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 25% |
| Medicine and Dentistry | 2 | 25% |
| Immunology and Microbiology | 1 | 13% |
| Unknown | 3 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 December 2014.
All research outputs
#20,245,139
of 22,772,779 outputs
Outputs from BMC Immunology
#500
of 585 outputs
Outputs of similar age
#303,053
of 361,775 outputs
Outputs of similar age from BMC Immunology
#15
of 15 outputs
Altmetric has tracked 22,772,779 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 585 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.