Title |
High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli
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Published in |
Microbial Cell Factories, October 2013
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DOI | 10.1186/1475-2859-12-97 |
Pubmed ID | |
Authors |
Kristof Zarschler, Stefanie Witecy, Franz Kapplusch, Christian Foerster, Holger Stephan |
Abstract |
For their application in the area of diagnosis and therapy, single-domain antibodies (sdAbs) offer multiple advantages over conventional antibodies and fragments thereof in terms of size, stability, solubility, immunogenicity, production costs as well as tumor uptake and blood clearance. Thus, sdAbs have been identified as valuable next-generation targeting moieties for molecular imaging and drug delivery in the past years. Since these probes are much less complex than conventional antibody fragments, bacterial expression represents a facile method in order to produce sdAbs in large amounts as soluble and functional proteins. |
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Geographical breakdown
Country | Count | As % |
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United States | 3 | 2% |
United Kingdom | 2 | 1% |
Brazil | 1 | <1% |
South Africa | 1 | <1% |
Japan | 1 | <1% |
China | 1 | <1% |
Unknown | 189 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 42 | 21% |
Researcher | 30 | 15% |
Student > Master | 29 | 15% |
Student > Bachelor | 19 | 10% |
Student > Doctoral Student | 14 | 7% |
Other | 23 | 12% |
Unknown | 41 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 63 | 32% |
Agricultural and Biological Sciences | 47 | 24% |
Chemistry | 10 | 5% |
Engineering | 10 | 5% |
Chemical Engineering | 7 | 4% |
Other | 23 | 12% |
Unknown | 38 | 19% |