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High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli

Overview of attention for article published in Microbial Cell Factories, October 2013
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (85th percentile)
  • High Attention Score compared to outputs of the same age and source (91st percentile)

Mentioned by

news
1 news outlet
patent
1 patent

Citations

dimensions_citation
72 Dimensions

Readers on

mendeley
198 Mendeley
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Title
High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli
Published in
Microbial Cell Factories, October 2013
DOI 10.1186/1475-2859-12-97
Pubmed ID
Authors

Kristof Zarschler, Stefanie Witecy, Franz Kapplusch, Christian Foerster, Holger Stephan

Abstract

For their application in the area of diagnosis and therapy, single-domain antibodies (sdAbs) offer multiple advantages over conventional antibodies and fragments thereof in terms of size, stability, solubility, immunogenicity, production costs as well as tumor uptake and blood clearance. Thus, sdAbs have been identified as valuable next-generation targeting moieties for molecular imaging and drug delivery in the past years. Since these probes are much less complex than conventional antibody fragments, bacterial expression represents a facile method in order to produce sdAbs in large amounts as soluble and functional proteins.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 198 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 2%
United Kingdom 2 1%
Brazil 1 <1%
South Africa 1 <1%
Japan 1 <1%
China 1 <1%
Unknown 189 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 42 21%
Researcher 30 15%
Student > Master 29 15%
Student > Bachelor 19 10%
Student > Doctoral Student 14 7%
Other 23 12%
Unknown 41 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 63 32%
Agricultural and Biological Sciences 47 24%
Chemistry 10 5%
Engineering 10 5%
Chemical Engineering 7 4%
Other 23 12%
Unknown 38 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 February 2022.
All research outputs
#3,415,510
of 25,374,917 outputs
Outputs from Microbial Cell Factories
#142
of 1,823 outputs
Outputs of similar age
#30,805
of 225,385 outputs
Outputs of similar age from Microbial Cell Factories
#1
of 23 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,823 research outputs from this source. They receive a mean Attention Score of 4.9. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 225,385 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.