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A novel loop-mediated isothermal amplification-based test for detecting Neospora caninum DNA

Overview of attention for article published in Parasites & Vectors, November 2017
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2 tweeters

Citations

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4 Dimensions

Readers on

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28 Mendeley
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Title
A novel loop-mediated isothermal amplification-based test for detecting Neospora caninum DNA
Published in
Parasites & Vectors, November 2017
DOI 10.1186/s13071-017-2549-y
Pubmed ID
Authors

Andrea Estefanía Ramos, Marina Muñoz, Jesús Alfredo Cortés-Vecino, Paola Barato, Manuel Alfonso Patarroyo

Abstract

Neospora caninum is a cyst-forming, coccidian parasite which is known to cause neurological disorders in dogs and abortion and neonatal mortality in cows and other livestock. This study reports the development of a loop-mediated isothermal amplification (LAMP) assay based on the Neospora caninum Nc-5 gene and compares its efficacy for detecting DNA to that of a semi-nested PCR test. Six primers were designed based on the Nc-5 repeat region of N. caninum. Specific LAMP primers led to successful amplification of N. caninum DNA at 63 °C in 30 min. The LAMP assay was highly specific (i.e. it did not reveal cross-reactivity with other parasite species) and had a low N. caninum plasmid DNA limit of detection (1 fg), which is ten times higher than that for the semi-nested PCR. LAMP applicability was evaluated using a set of naturally-infected samples (59 from canine faeces and five from bovine abortions). Thirty-nine percent (25/64) of the naturally-infected samples were positive for N. caninum DNA by LAMP and 36% (23/64) by semi-nested PCR. However, the LAMP assay is much faster to perform than semi-nested PCR and provides results in 30 min. The optimized reaction conditions described in this study resulted in a sensitive, specific and rapid technique for detecting N. caninum DNA. Considering the advantages of LAMP for detecting N. caninum DNA, further assays aimed at testing its usefulness on a wider range of field samples are recommended.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 14%
Student > Bachelor 3 11%
Student > Master 3 11%
Student > Ph. D. Student 3 11%
Student > Doctoral Student 3 11%
Other 5 18%
Unknown 7 25%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 7 25%
Agricultural and Biological Sciences 4 14%
Biochemistry, Genetics and Molecular Biology 4 14%
Immunology and Microbiology 1 4%
Social Sciences 1 4%
Other 1 4%
Unknown 10 36%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 November 2017.
All research outputs
#9,379,703
of 12,225,951 outputs
Outputs from Parasites & Vectors
#2,025
of 3,095 outputs
Outputs of similar age
#221,996
of 339,289 outputs
Outputs of similar age from Parasites & Vectors
#127
of 216 outputs
Altmetric has tracked 12,225,951 research outputs across all sources so far. This one is in the 20th percentile – i.e., 20% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,095 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 25th percentile – i.e., 25% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 339,289 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 216 others from the same source and published within six weeks on either side of this one. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.