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Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalisin dairy cattle in Ningxia, northwestern China

Overview of attention for article published in BMC Veterinary Research, December 2014
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Title
Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalisin dairy cattle in Ningxia, northwestern China
Published in
BMC Veterinary Research, December 2014
DOI 10.1186/s12917-014-0292-6
Pubmed ID
Authors

Jianying Huang, Daoyou Yue, Meng Qi, Rongjun Wang, Jinfeng Zhao, Junqiang Li, Ke Shi, Ming Wang, Longxian Zhang

Abstract

Background Cryptosporidium spp. and Giardia duodenalis are important gastrointestinal protists in humans and animals worldwide. In China, bovine cryptosporidiosis and giardiasis are of increasing concern because cattle are important reservoirs of these parasites, which have become potential threats to public health and to large numbers of cattle in recent years.ResultsA total of 1366 fecal samples from the Ningxia Autonomous Region were examined. The overall infection rates for Cryptosporidium spp. and G. duodenalis were 1.61% and 2.12%, respectively. Cryptosporidium was only detected in preweaned calves and adults older than 2 years, whereas G. duodenalis was only detected in calves aged less than 11 months. Cryptosporidium spp. were characterized with a PCR¿restriction fragment length polymorphism analysis and DNA sequence analysis of the small subunit rRNA gene. Three Cryptosporidium species were identified: C. parvum (n =15) and C. bovis (n =4) in preweaned calves, and C. andersoni (n =4) in adults aged over 2 years. A DNA sequence analysis of the gp60 gene suggested that the 15 C. parvum isolates all belonged to subtype IIdA15G1. Twenty-nine G. duodenalis isolates were analyzed by DNA sequencing of the triosephosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes. Two G. duodenalis assemblages were identified, assemblages E (n =15) and B (n =4, one subtype B1 and three subtype B2) in preweaned calves, and assemblage E (n =10) in 3¿11-month-old calves.ConclusionsThe predominance of C. parvum detected in preweaned calves and the first identified subtype IIdA15G1 in dairy cattle, and the dominant G. duodenalis assemblage E in this study differed considerably from those found in Henan, Heilongjiang, and Shannxi Provinces. Our findings further confirm the dominance of C. parvum IId subtypes in China.

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Mendeley readers

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The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Mexico 1 2%
Unknown 44 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 18%
Student > Bachelor 6 13%
Student > Ph. D. Student 5 11%
Student > Master 4 9%
Lecturer 3 7%
Other 9 20%
Unknown 10 22%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 9 20%
Agricultural and Biological Sciences 6 13%
Biochemistry, Genetics and Molecular Biology 5 11%
Medicine and Dentistry 5 11%
Environmental Science 1 2%
Other 5 11%
Unknown 14 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 December 2014.
All research outputs
#18,386,678
of 22,774,233 outputs
Outputs from BMC Veterinary Research
#1,918
of 3,045 outputs
Outputs of similar age
#261,452
of 361,040 outputs
Outputs of similar age from BMC Veterinary Research
#57
of 95 outputs
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