Title |
The case for decoupling assembly and submission standards to maintain a more flexible registry of biological parts
|
---|---|
Published in |
Journal of Biological Engineering, December 2014
|
DOI | 10.1186/1754-1611-8-28 |
Pubmed ID | |
Authors |
Razan N Alnahhas, Ben Slater, Yunle Huang, Catherine Mortensen, Jordan W Monk, Yousef Okasheh, Marco D Howard, Neil R Gottel, Michael J Hammerling, Jeffrey E Barrick |
Abstract |
The Registry of Standard Biological Parts only accepts genetic parts compatible with the RFC 10 BioBrick format. This combined assembly and submission standard requires that four unique restriction enzyme sites must not occur in the DNA sequence encoding a part. We present evidence that this requirement places a nontrivial burden on iGEM teams developing large and novel parts. We further argue that the emergence of inexpensive DNA synthesis and versatile assembly methods reduces the utility of coupling submission and assembly standards and propose a submission standard that is compatible with current quality control strategies while nearly eliminating sequence constraints on submitted parts. |
X Demographics
Geographical breakdown
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United States | 8 | 62% |
Spain | 1 | 8% |
Venezuela, Bolivarian Republic of | 1 | 8% |
Unknown | 3 | 23% |
Demographic breakdown
Type | Count | As % |
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Scientists | 7 | 54% |
Members of the public | 6 | 46% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 14% |
United Kingdom | 1 | 4% |
Spain | 1 | 4% |
China | 1 | 4% |
Unknown | 21 | 75% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 10 | 36% |
Student > Ph. D. Student | 8 | 29% |
Student > Bachelor | 6 | 21% |
Professor | 2 | 7% |
Other | 1 | 4% |
Other | 1 | 4% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 18 | 64% |
Biochemistry, Genetics and Molecular Biology | 4 | 14% |
Social Sciences | 2 | 7% |
Nursing and Health Professions | 1 | 4% |
Immunology and Microbiology | 1 | 4% |
Other | 1 | 4% |
Unknown | 1 | 4% |