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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Biofilm formation by virulent and non-virulent strains of Haemophilus parasuis
|
|---|---|
| Published in |
Veterinary Research, November 2014
|
| DOI | 10.1186/s13567-014-0104-9 |
| Pubmed ID | |
| Authors |
Bernardo Bello-Ortí, Vincent Deslandes, Yannick DN Tremblay, Josée Labrie, Kate J Howell, Alexander W Tucker, Duncan J Maskell, Virginia Aragon, Mario Jacques |
| Abstract |
Haemophilus parasuis is a commensal bacterium of the upper respiratory tract of healthy pigs. It is also the etiological agent of Glässer's disease, a systemic disease characterized by polyarthritis, fibrinous polyserositis and meningitis, which causes high morbidity and mortality in piglets. The aim of this study was to evaluate biofilm formation by well-characterized virulent and non-virulent strains of H. parasuis. We observed that non-virulent strains isolated from the nasal cavities of healthy pigs formed significantly (p < 0.05) more biofilms than virulent strains isolated from lesions of pigs with Glässer's disease. These differences were observed when biofilms were formed in microtiter plates under static conditions or formed in the presence of shear force in a drip-flow apparatus or a microfluidic system. Confocal laser scanning microscopy using different fluorescent probes on a representative subset of strains indicated that the biofilm matrix contains poly-N-acetylglucosamine, proteins and eDNA. The biofilm matrix was highly sensitive to degradation by proteinase K. Comparison of transcriptional profiles of biofilm and planktonic cells of the non-virulent H. parasuis F9 strain revealed a significant number of up-regulated membrane-related genes in biofilms, and genes previously identified in Actinobacillus pleuropneumoniae biofilms. Our data indicate that non-virulent strains of H. parasuis have the ability to form robust biofilms in contrast to virulent, systemic strains. Biofilm formation might therefore allow the non-virulent strains to colonize and persist in the upper respiratory tract of pigs. Conversely, the planktonic state of the virulent strains might allow them to disseminate within the host. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 55 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Russia | 1 | 2% |
| Thailand | 1 | 2% |
| Unknown | 52 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 11 | 20% |
| Student > Ph. D. Student | 10 | 18% |
| Researcher | 8 | 15% |
| Other | 6 | 11% |
| Student > Doctoral Student | 5 | 9% |
| Other | 5 | 9% |
| Unknown | 10 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 22 | 40% |
| Veterinary Science and Veterinary Medicine | 7 | 13% |
| Biochemistry, Genetics and Molecular Biology | 5 | 9% |
| Medicine and Dentistry | 4 | 7% |
| Immunology and Microbiology | 3 | 5% |
| Other | 4 | 7% |
| Unknown | 10 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 December 2014.
All research outputs
#20,656,161
of 25,374,647 outputs
Outputs from Veterinary Research
#1,035
of 1,337 outputs
Outputs of similar age
#274,493
of 369,398 outputs
Outputs of similar age from Veterinary Research
#15
of 20 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,337 research outputs from this source. They receive a mean Attention Score of 5.0. This one is in the 10th percentile – i.e., 10% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 369,398 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.