Title |
Generation in yeast of recombinant virus-like particles of porcine circovirus type 2 capsid protein and their use for a serologic assay and development of monoclonal antibodies
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Published in |
BMC Biotechnology, December 2014
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DOI | 10.1186/s12896-014-0100-1 |
Pubmed ID | |
Authors |
Juozas Nainys, Rita Lasickiene, Rasa Petraityte-Burneikiene, Jonas Dabrisius, Raimundas Lelesius, Vilimas Sereika, Aurelija Zvirbliene, Kestutis Sasnauskas, Alma Gedvilaite |
Abstract |
BackgroundPorcine circovirus type 2 (PCV2) is considered to be an important emerging pathogen associated with a number of different syndromes and diseases in pigs known as PCV2-associated diseases. It has been responsible for significant mortality among pigs and remains a serious economic problem to the swine industry worldwide leading to significant negative impacts on profitability of pork production.ResultsIn this study we have demonstrated that PCV2 capsid (Cap) protein based virus-like particles (VLPs) were efficiently produced in yeast S. cerevisiae and induced production of monoclonal antibodies (MAbs) reactive with virus-infected cells. Moreover, PCV2 Cap VLPs served as a highly specific recombinant antigen for the development of an indirect IgG PCV2 Cap VLP-based ELISA for the detection of virus-specific IgG antibodies in swine sera. Four hundred-nine serum samples collected from pigs in Lithuania were tested for PCV2-specific IgG to determine the sensitivity and specificity of the newly developed ELISA in parallel using a commercial SERELISA test as a gold standard. From 409 tested serum samples, 297 samples were positive by both assays. Thirty-nine sera from 112 serum samples were determined as negative by SERELISA but were found to be positive both in the newly developed indirect IgG PCV2 Cap VLP-based ELISA and the PCR test.ConclusionsWe have demonstrated that S. cerevisiae expression system is an alternative to insect/baculovirus expression system for production of homogenous in size and shape PCV2 Cap protein-based VLPs similar to native virions. Yeast expression system tolerated native virus genes encoding PCV2 Cap protein variants as well as the codon-optimized gene. Moreover, yeast-derived PCV2 Cap VLPs were capable to induce the generation of PCV2-specific MAbs that did not show any cross-reactivity with PCV1-infected cells. The high sensitivity and specificity of the indirect IgG PCV2 Cap VLP-based ELISA clearly suggested that this assay is potentially useful diagnostic tool for screening PCV2¿suspected samples. |
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Unknown | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
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Denmark | 1 | 2% |
Unknown | 47 | 98% |
Demographic breakdown
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Researcher | 14 | 29% |
Student > Master | 9 | 19% |
Student > Ph. D. Student | 6 | 13% |
Student > Bachelor | 3 | 6% |
Professor | 3 | 6% |
Other | 6 | 13% |
Unknown | 7 | 15% |
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Agricultural and Biological Sciences | 14 | 29% |
Biochemistry, Genetics and Molecular Biology | 11 | 23% |
Veterinary Science and Veterinary Medicine | 7 | 15% |
Immunology and Microbiology | 4 | 8% |
Medicine and Dentistry | 3 | 6% |
Other | 2 | 4% |
Unknown | 7 | 15% |