Title |
Construction of a high-density genetic map based on large-scale markers developed by specific length amplified fragment sequencing (SLAF-seq) and its application to QTL analysis for isoflavone content in Glycine max
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Published in |
BMC Genomics, December 2014
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DOI | 10.1186/1471-2164-15-1086 |
Pubmed ID | |
Authors |
Bin Li, Ling Tian, Jingying Zhang, Long Huang, Fenxia Han, Shurong Yan, Lianzheng Wang, Hongkun Zheng, Junming Sun |
Abstract |
Quantitative trait locus (QTL) mapping is an efficient approach to discover the genetic architecture underlying complex quantitative traits. However, the low density of molecular markers in genetic maps has limited the efficiency and accuracy of QTL mapping. In this study, specific length amplified fragment sequencing (SLAF-seq), a new high-throughput strategy for large-scale SNP discovery and genotyping based on next generation sequencing (NGS), was employed to construct a high-density soybean genetic map using recombinant inbred lines (RILs, Luheidou2 × Nanhuizao, F5:8). With this map, the consistent QTLs for isoflavone content across various environments were identified. |
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