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Attention Score in Context
| Title |
Functional analysis of Flavonoid 3′,5′-hydroxylase from Tea plant (Camellia sinensis): critical role in the accumulation of catechins
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|---|---|
| Published in |
BMC Plant Biology, December 2014
|
| DOI | 10.1186/s12870-014-0347-7 |
| Pubmed ID | |
| Authors |
Yun-Sheng Wang, Yu-Jiao Xu, Li-Ping Gao, Oliver Yu, Xin-Zhen Wang, Xiu-Juan He, Xiao-Lan Jiang, Ya-Jun Liu, Tao Xia |
| Abstract |
BackgroundFlavonoid 3¿,5¿-hydroxylase (F3¿5¿H), an important branch point enzyme in tea plant flavan-3-ol synthesis, belongs to the CYP75A subfamily and catalyzes the conversion of flavones, flavanones, dihydroflavonols and flavonols into 3¿,4¿,5¿-hydroxylated derivatives. However, whether B-ring hydroxylation occurs at the level of flavanones and/or dihydroflavonols, in vivo remains unknown.ResultsThe Camellia sinensis F3¿5¿H (CsF3¿5¿H) gene was isolated from tea cDNA library. Expression pattern analysis revealed that CsF3¿5¿H expression was tissue specific, very high in the buds and extremely low in the roots. CsF3¿5¿H expression was enhanced by light and sucrose. Over-expression of CsF3¿5¿H produced new-delphinidin derivatives, and increased the cyanidin derivative content of corollas of transgenic tobacco plants, resulting in the deeper transgenic plant flower color. Heterologous expressions of CsF3¿5¿H in yeast were carried out to demonstrate the function of CsF3¿5¿H enzyme in vitro. Heterologous expression of the modified CsF3¿5¿H (CsF3¿5¿H gene fused with Vitis vinifera signal peptide, FSI) revealed that 4¿-hydroxylated flavanone (naringenin, N) is the optimum substrate for CsF3¿5¿H, and was efficiently converted into both 3¿4¿- and 3¿4¿5¿-forms. The ratio of 3¿4¿5¿- to 3¿4¿-hydroxylated products in FSI transgenic cells was significantly higher than VvF3¿5¿H cells.ConclusionsCsF3¿5¿H is a key controller of tri-hydroxyl flavan-3-ol synthesis in tea plants, which can effectively convert 4¿-hydroxylated flavanone into 3¿4¿5¿- and/or 3¿4¿-hydroxylated products. These findings provide an important basis for further studies of flavonoid biosynthesis in tea plants. Such studies would help accelerate flavonoid metabolic engineering in order to increase B-ring tri-hydroxyl product yields. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 73 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 1% |
| Australia | 1 | 1% |
| Unknown | 71 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 21% |
| Student > Master | 10 | 14% |
| Student > Bachelor | 10 | 14% |
| Student > Doctoral Student | 4 | 5% |
| Researcher | 4 | 5% |
| Other | 15 | 21% |
| Unknown | 15 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 28 | 38% |
| Biochemistry, Genetics and Molecular Biology | 10 | 14% |
| Medicine and Dentistry | 5 | 7% |
| Chemistry | 4 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 1% |
| Other | 4 | 5% |
| Unknown | 21 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 September 2021.
All research outputs
#6,945,280
of 22,774,233 outputs
Outputs from BMC Plant Biology
#550
of 3,237 outputs
Outputs of similar age
#97,597
of 361,216 outputs
Outputs of similar age from BMC Plant Biology
#26
of 99 outputs
Altmetric has tracked 22,774,233 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 3,237 research outputs from this source. They receive a mean Attention Score of 3.0. This one has done well, scoring higher than 82% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 361,216 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 99 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.