Title |
Quantifying CD4 receptor protein in two human CD4+ lymphocyte preparations for quantitative flow cytometry
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Published in |
Clinical Proteomics, December 2014
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DOI | 10.1186/1559-0275-11-43 |
Pubmed ID | |
Authors |
Meiyao Wang, Martin Misakian, Hua-Jun He, Peter Bajcsy, Fatima Abbasi, Jeffrey M Davis, Kenneth D Cole, Illarion V Turko, Lili Wang |
Abstract |
In our previous study that characterized different human CD4+ lymphocyte preparations, it was found that both commercially available cryopreserved peripheral blood mononuclear cells (PBMC) and a commercially available lyophilized PBMC (Cyto-Trol™) preparation fulfilled a set of criteria for serving as biological calibrators for quantitative flow cytometry. However, the biomarker CD4 protein expression level measured for T helper cells from Cyto-Trol was about 16% lower than those for cryopreserved PBMC and fresh whole blood using flow cytometry and mass cytometry. A primary reason was hypothesized to be due to steric interference in anti- CD4 antibody binding to the smaller sized lyophilized control cells. |
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