Title |
A genome-guided analysis of energy conservation in the thermophilic, cytochrome-free acetogenic bacterium Thermoanaerobacter kivui
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Published in |
BMC Genomics, December 2014
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DOI | 10.1186/1471-2164-15-1139 |
Pubmed ID | |
Authors |
Verena Hess, Anja Poehlein, Marie Charlotte Weghoff, Rolf Daniel, Volker Müller |
Abstract |
Acetogenic bacteria are able to use CO2 as terminal electron acceptor of an anaerobic respiration, thereby producing acetate with electrons coming from H2. Due to this feature, acetogens came into focus as platforms to produce biocommodities from waste gases such as H2 + CO2 and/or CO. A prerequisite for metabolic engineering is a detailed understanding of the mechanisms of ATP synthesis and electron-transfer reactions to ensure redox homeostasis. Acetogenesis involves the reduction of CO2 to acetate via soluble enzymes and is coupled to energy conservation by a chemiosmotic mechanism. The membrane-bound module, acting as an ion pump, was of special interest for decades and recently, an Rnf complex was shown to couple electron flow from reduced ferredoxin to NAD+ with the export of Na+ in Acetobacterium woodii. However, not all acetogens have rnf genes in their genome. In order to gain further insights into energy conservation of non-Rnf-containing, thermophilic acetogens, we sequenced the genome of Thermoanaerobacter kivui. |
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Unknown | 2 | 50% |
Demographic breakdown
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Mendeley readers
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Student > Postgraduate | 5 | 6% |
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Chemical Engineering | 3 | 4% |
Other | 13 | 16% |
Unknown | 15 | 18% |