Title |
Validation of miRNA genes suitable as reference genes in qPCR analyses of miRNA gene expression in Atlantic salmon (Salmo salar)
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Published in |
BMC Research Notes, December 2014
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DOI | 10.1186/1756-0500-7-945 |
Pubmed ID | |
Authors |
Ilona Johansen, Rune Andreassen |
Abstract |
MicroRNAs (miRNAs) are an abundant class of endogenous small RNA molecules that downregulate gene expression at the post-transcriptional level. They play important roles by regulating genes that control multiple biological processes, and recent years there has been an increased interest in studying miRNA genes and miRNA gene expression. The most common method applied to study gene expression of single genes is quantitative PCR (qPCR). However, before expression of mature miRNAs can be studied robust qPCR methods (miRNA-qPCR) must be developed. This includes identification and validation of suitable reference genes. We are particularly interested in Atlantic salmon (Salmo salar). This is an economically important aquaculture species, but no reference genes dedicated for use in miRNA-qPCR methods has been validated for this species. Our aim was, therefore, to identify suitable reference genes for miRNA-qPCR methods in Salmo salar. |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 5 | 19% |
Student > Ph. D. Student | 4 | 15% |
Student > Master | 4 | 15% |
Professor > Associate Professor | 3 | 12% |
Student > Bachelor | 2 | 8% |
Other | 4 | 15% |
Unknown | 4 | 15% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 10 | 38% |
Biochemistry, Genetics and Molecular Biology | 5 | 19% |
Unspecified | 1 | 4% |
Nursing and Health Professions | 1 | 4% |
Immunology and Microbiology | 1 | 4% |
Other | 2 | 8% |
Unknown | 6 | 23% |