Title |
Uptake and cytotoxicity of citrate-coated gold nanospheres: Comparative studies on human endothelial and epithelial cells
|
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Published in |
Particle and Fibre Toxicology, July 2012
|
DOI | 10.1186/1743-8977-9-23 |
Pubmed ID | |
Authors |
Christian Freese, Chiara Uboldi, Matthew I Gibson, Ronald E Unger, Babette B Weksler, Ignacio A Romero, Pierre-Olivier Couraud, C James Kirkpatrick |
Abstract |
The use of gold nanoparticles (AuNPs) for diagnostic applications and for drug and gene-delivery is currently under intensive investigation. For such applications, biocompatibility and the absence of cytotoxicity of AuNPs is essential. Although generally considered as highly biocompatible, previous in vitro studies have shown that cytotoxicity of AuNPs in certain human epithelial cells was observed. In particular, the degree of purification of AuNPs (presence of sodium citrate residues on the particles) was shown to affect the proliferation and induce cytotoxicity in these cells. To expand these studies, we have examined if the effects are related to nanoparticle size (10, 11 nm, 25 nm), to the presence of sodium citrate on the particles' surface or they are due to a varying degree of internalization of the AuNPs. Since two cell types are present in the major barriers to the outside in the human body, we have also included endothelial cells from the vasculature and blood brain barrier. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Samoa | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
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Portugal | 1 | <1% |
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Student > Master | 29 | 16% |
Student > Bachelor | 20 | 11% |
Researcher | 18 | 10% |
Student > Doctoral Student | 12 | 7% |
Other | 26 | 15% |
Unknown | 28 | 16% |
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Engineering | 9 | 5% |
Materials Science | 8 | 5% |
Other | 37 | 21% |
Unknown | 37 | 21% |