You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
X Demographics
Mendeley readers
Attention Score in Context
| Title |
MEK/ERK signaling pathway is required for enterovirus 71 replication in immature dendritic cells
|
|---|---|
| Published in |
Virology Journal, December 2014
|
| DOI | 10.1186/s12985-014-0227-7 |
| Pubmed ID | |
| Authors |
Weifeng Shi, Xueling Hou, Hongjun Peng, Li Zhang, Yuanyuan Li, Zhiwen Gu, Qingbo Jiang, Mei Shi, Yun Ji, Jingting Jiang |
| Abstract |
BackgroundThe mitogen-activated protein kinase kinase/extracellular-signal-regulated kinase (MEK/ERK) signaling pathway is involved in viral life cycle. However, the effect of MEK/ERK pathway in enterovirus 71(EV71)-infected immature dendritic cells (iDCs) is still unclear.MethodsHuman peripheral blood mononuclear cells (PBMCs) were isolated and induced to generate iDCs. Unifected iDCs and EV71-infected iDCs with a multiplicity of infection (MOI¿=¿5) were analyzed by flow cytometry. Differential gene expressions of MEK/ERK signaling pathway molecules in EV71-infected iDCs were performed by PCR arrays. The phosphorylation of MEK/ERK pathway molecules in EV71-infected iDCs preincubated without or with U0126 (20 ¿M) at indicated times was detected by Western blot. The concentrations of IL-1¿, IL-2, IL-6, IL-12, TNF-¿, IFN-¿1, IFN-ß and IFN-¿ in culture supernatant were analyzed by the luminex fluorescent technique.ResultsWhen iDCs were infected with EV71 for 24 h, the percentage of CD80, CD83, CD86 and HLA-DR expressed on iDCs significantly increased. PCR arrays showed that gene expressions of molecules in MEK/ERK signaling pathway were remarkably upregulated in EV71-infected iDCs. EV71 infection activated both MEK1/2 and ERK1/2, which phosphorylated their downstream transcription factor c-Fos, c-Jun, c-myc and Elk1. Importantly, the treatment of U0126 significantly inhibited MEK/ERK signaling pathway molecules and severely impaired virus replication., Additionally, EV71 infection promoted the expression of son of sevenless (SOS1) and increased the secretion of IL-1¿, IL-2, IL-6, IL-12, TNF-¿,IFN-ß and IFN-¿. Furthermore,the release of IL-1¿, IL-2,IL-6 and TNF-¿ could be effectively suppressed by inhibitor U0126.ConclusionsOur data suggest that the MEK/ERK signaling pathway plays an important role in EV71-infected iDCs and these molecules may be potential targets for the development of new anti-EV71 drugs. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 20% |
| United Kingdom | 1 | 20% |
| Unknown | 3 | 60% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 2 | 40% |
| Members of the public | 2 | 40% |
| Practitioners (doctors, other healthcare professionals) | 1 | 20% |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 24 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 25% |
| Researcher | 5 | 21% |
| Student > Doctoral Student | 2 | 8% |
| Student > Master | 2 | 8% |
| Student > Bachelor | 1 | 4% |
| Other | 3 | 13% |
| Unknown | 5 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 29% |
| Biochemistry, Genetics and Molecular Biology | 4 | 17% |
| Medicine and Dentistry | 4 | 17% |
| Immunology and Microbiology | 3 | 13% |
| Psychology | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 5 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 October 2015.
All research outputs
#7,205,489
of 22,775,504 outputs
Outputs from Virology Journal
#849
of 3,041 outputs
Outputs of similar age
#100,429
of 352,738 outputs
Outputs of similar age from Virology Journal
#25
of 68 outputs
Altmetric has tracked 22,775,504 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 3,041 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.7. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,738 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 68 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.