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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Identification of putative interactions between swine and human influenza A virus nucleoprotein and human host proteins
|
|---|---|
| Published in |
Virology Journal, December 2014
|
| DOI | 10.1186/s12985-014-0228-6 |
| Pubmed ID | |
| Authors |
Alex Generous, Molly Thorson, Jeff Barcus, Joseph Jacher, Marc Busch, Heidi Sleister |
| Abstract |
BackgroundInfluenza A viruses (IAVs) are important pathogens that affect the health of humans and many additional animal species. IAVs are enveloped, negative single-stranded RNA viruses whose genome encodes at least ten proteins. The IAV nucleoprotein (NP) is a structural protein that associates with the viral RNA and is essential for virus replication. Understanding how IAVs interact with host proteins is essential for elucidating all of the required processes for viral replication, restrictions in species host range, and potential targets for antiviral therapies.MethodsIn this study, the NP from a swine IAV was cloned into a yeast two-hybrid ¿bait¿ vector for expression of a yeast Gal4 binding domain (BD)-NP fusion protein. This ¿bait¿ was used to screen a Y2H human HeLa cell ¿prey¿ library which consisted of human proteins fused to the Gal4 protein¿s activation domain (AD). The interaction of ¿bait¿ and ¿prey¿ proteins resulted in activation of reporter genes.ResultsSeventeen positive bait-prey interactions were isolated in yeast. All of the ¿prey¿ isolated also interact in yeast with a NP ¿bait¿ cloned from a human IAV strain. Isolation and sequence analysis of the cDNAs encoding the human prey proteins revealed ten different human proteins. These host proteins are involved in various host cell processes and structures, including purine biosynthesis (PAICS), metabolism (ACOT13), proteasome (PA28B), DNA-binding (MSANTD3), cytoskeleton (CKAP5), potassium channel formation (KCTD9), zinc transporter function (SLC30A9), Na+/K+ ATPase function (ATP1B1), and RNA splicing (TRA2B).ConclusionsTen human proteins were identified as interacting with IAV NP in a Y2H screen. Some of these human proteins were reported in previous screens aimed at elucidating host proteins relevant to specific viral life cycle processes such as replication. This study extends previous findings by suggesting a mechanism by which these host proteins associate with the IAV, i.e., physical interaction with NP. Furthermore, this study revealed novel host protein-NP interactions in yeast. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Science communicators (journalists, bloggers, editors) | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 37 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 35% |
| Professor | 4 | 11% |
| Researcher | 4 | 11% |
| Student > Bachelor | 3 | 8% |
| Student > Master | 3 | 8% |
| Other | 6 | 16% |
| Unknown | 4 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 30% |
| Biochemistry, Genetics and Molecular Biology | 8 | 22% |
| Medicine and Dentistry | 6 | 16% |
| Immunology and Microbiology | 3 | 8% |
| Chemistry | 2 | 5% |
| Other | 3 | 8% |
| Unknown | 4 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 April 2015.
All research outputs
#12,714,958
of 22,775,504 outputs
Outputs from Virology Journal
#1,159
of 3,041 outputs
Outputs of similar age
#161,138
of 352,738 outputs
Outputs of similar age from Virology Journal
#30
of 68 outputs
Altmetric has tracked 22,775,504 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,041 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.7. This one has gotten more attention than average, scoring higher than 60% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,738 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 68 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.