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Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues

Overview of attention for article published in Plant Methods, December 2017
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  • Above-average Attention Score compared to outputs of the same age (51st percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

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4 tweeters

Citations

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1 Dimensions

Readers on

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21 Mendeley
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Title
Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues
Published in
Plant Methods, December 2017
DOI 10.1186/s13007-017-0257-4
Pubmed ID
Authors

Muhammad Waqar Hameed, Ilona Juszczak, Ralph Bock, Joost Thomas van Dongen

Abstract

To investigate translational regulation of gene expression in plant mitochondria, a mitochondrial polysome isolation protocol was established for tobacco to investigate polysomal mRNA loading as a proxy for translational activity. Furthermore, we developed an oligonucleotide based microarray platform to determine the level of Nicotiana tabacum and Arabidopsis thaliana mitochondrial mRNA. Microarray analysis of free and polysomal mRNAs was used to characterize differences in the levels of free transcripts and ribosome-bound mRNAs in various organs of tobacco plants. We have observed higher mitochondrial transcript levels in young leaves, flowers and floral buds as compared to fully expanded leaves and roots. A similar pattern of abundance was observed for ribosome-bound mitochondrial mRNAs in these tissues. However, the accumulation of the mitochondrial protein COX2 was found to be inversely related to that of its ribosome-bound mRNA. Our results indicate that the association of mitochondrial mRNAs to ribosomes is largely determined by the total transcript level of a gene. However, at least for Cox2, we demonstrated that the level of ribosome-bound mRNA is not reflected by the amount of COX2 protein.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Professor 3 14%
Researcher 3 14%
Student > Bachelor 3 14%
Student > Master 3 14%
Student > Ph. D. Student 3 14%
Other 5 24%
Unknown 1 5%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 38%
Agricultural and Biological Sciences 8 38%
Medicine and Dentistry 1 5%
Neuroscience 1 5%
Chemistry 1 5%
Other 0 0%
Unknown 2 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 December 2017.
All research outputs
#10,181,890
of 17,635,820 outputs
Outputs from Plant Methods
#497
of 849 outputs
Outputs of similar age
#196,860
of 418,529 outputs
Outputs of similar age from Plant Methods
#47
of 89 outputs
Altmetric has tracked 17,635,820 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 849 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 418,529 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.
We're also able to compare this research output to 89 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.