Title |
Graphene-based dental adhesive with anti-biofilm activity
|
---|---|
Published in |
Journal of Nanobiotechnology, December 2017
|
DOI | 10.1186/s12951-017-0322-1 |
Pubmed ID | |
Authors |
Agnese Bregnocchi, Elena Zanni, Daniela Uccelletti, Fabrizio Marra, Domenico Cavallini, Francesca De Angelis, Giovanni De Bellis, Maurizio Bossù, Gaetano Ierardo, Antonella Polimeni, Maria Sabrina Sarto |
Abstract |
Secondary caries are considered the main cause of dental restoration failure. In this context, anti-biofilm and bactericidal properties are desired in dental materials against pathogens such as Streptococcus mutans. To this purpose, graphene based materials can be used as fillers of polymer dental adhesives. In this work, we investigated the possibility to use as filler of dental adhesives, graphene nanoplatelets (GNP), a non toxic hydrophobic nanomaterial with antimicrobial and anti-biofilm properties. Graphene nanoplatelets have been produced starting from graphite intercalated compounds through a process consisting of thermal expansion and liquid exfoliation. Then, a dental adhesive filled with GNPs at different volume fractions has been produced through a solvent evaporation method. The rheological properties of the new experimental adhesives have been assessed experimentally. The adhesive properties have been tested using microtensile bond strength measurements (µ-TBS). Biocidal activity has been studied using the colony forming units count (CFU) method. The anti-biofilm properties have been demonstrated through FE-SEM imaging of the biofilm development after 3 and 24 h of growth. A significantly lower vitality of S. mutans cells has been demonstrated when in contact with the GNP filled dental adhesives. Biofilm growth on adhesive-covered dentine tissues demonstrated anti-adhesion properties of the produced materials. µ-TBS results demonstrated no significant difference in µ-TBS between the experimental and the control adhesive. The rheology tests highlighted the necessity to avoid low shear rate regimes during adhesive processing and application in clinical protocol, and confirmed that the adhesive containing the 0.2%wt of GNPs possess mechanical properties comparable with the ones of the control adhesive. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 134 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 19 | 14% |
Student > Ph. D. Student | 15 | 11% |
Researcher | 13 | 10% |
Student > Bachelor | 12 | 9% |
Student > Doctoral Student | 8 | 6% |
Other | 16 | 12% |
Unknown | 51 | 38% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 34 | 25% |
Materials Science | 10 | 7% |
Agricultural and Biological Sciences | 7 | 5% |
Biochemistry, Genetics and Molecular Biology | 4 | 3% |
Chemistry | 4 | 3% |
Other | 22 | 16% |
Unknown | 53 | 40% |